PickMutant™ Site-directed Mutagenesis Kit
Efficient site-directed mutagenesis kit for introducing precise point mutations, deletions, or insertions.
PickMutant™ Site-Directed Mutagenesis Kit enables the generation of specific DNA mutations with high efficiency and accuracy. It simplifies the process of introducing base substitutions, insertions, or deletions.
The kit provides all necessary reagents for PCR-based mutagenesis and ensures reproducible results for molecular cloning and gene function studies.
Have questions? Contact us
Price: 110.90 €
Detailed information:
Advantages & Features
-
Highly effective: point mutations, deletions, and insertions.
-
Fast workflow: <3 h single-step protocol.
-
Versatile: compatible with multiple vectors.
-
Robust: simultaneous PCR assembly and cloning.
Specifications
Includes
– 150 μL Proofreading DNA Polymerase MasterMix (2x)
– 300 U Glue Enzyme (10 U/μL)
– 40 μL Glue Enzyme Buffer (10x)
– 5 μL Insert Control DNA
– 15 μL pSpark® I (20 ng/μL)
Download documentation
Datasheet MSDSApplications
-
Site-directed mutagenesis.
-
Protein functional analysis.
-
Identification of enzyme active sites.
-
Protein design and synthetic biology.
Tables & Figures
Quality Control
-
Tested using provided control DNA insert.
Advice
-
Use high-quality primers for best efficiency.
-
Handle reagents with nuclease-free practices.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
- Tee, K. L., & Wong, T. S. (2017). Back to Basics: Creating Genetic Diversity. In Directed Enzyme Evolution: Advances and Applications (pp. 201-227). Springer, Cham.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
Site-directed mutagenesis kit, Targeted DNA mutagenesis kit, Point mutation PCR kit, Directed mutagenesis kit, PCR-based mutagenesis kit
Also known as:
- Spanish: Kit de mutagénesis dirigida, Mutagénesis puntual por PCR, Kit de mutación específica
- French: Kit de mutagénèse dirigée, Mutagénèse ponctuelle par PCR, Kit de mutation ciblée
- German: Kit für gerichtete Mutagenese, Punktmutations-PCR, Zielgerichtetes Mutagenesekit
- Italian: Kit mutagenesi sito-diretta, Mutagenesi puntuale PCR, Kit mutazione mirata
FAQs
Q: How long does the protocol take?
A: Less than 3 hours, single-step procedure.
Q: Can it be used with any vector?
A: Yes, compatible with pSpark® or alternative cloning vectors.
Q: What mutations can be introduced?
A: Point mutations, deletions, and insertions.
Claire Dubois –
We used this kit for introducing point mutations into expression plasmids for protein engineering work. The protocol was easy to follow and reduced hands-on time compared to our previous workflow. It worked well for routine construct verification projects.