PRImeDETECT™ Listeria monocytogenes Detection Kit

• Amplification limit: 10 UG per reaction (100%), 2 UG per reaction (40%)
• Quantification limit:  20 UG per reaction (100%).
• Quantification Dynamic range: 6 logs
• Target: Listeria monocytogenes
• Inclusivity: Positive in 48 reference strains of L. monocytogenes (serovars 1 to 7) from different collections.
• Exclusivity:  100% Tested with 94 nontarget strains composed of 51 strains of Listeria nomonocytogenes and 43 strains of non-Listeria.
• Detection: Probe labelled with fluorescent dyes Listeria monocytogenes: FAM-BHQ1a; IAC: HEXBHQ1.
• Thermal Cycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers.

– 1 x PCR MasterMix
– 1 x DNAready Lysis Buffer
– 1 x PCR Positive Control
– 1 x PCR Negative Control

• Detection of Listeria monocytogenes in food samples.

• Environmental monitoring for Listeria contamination.

• Food safety and quality control programs.

• Rapid microbiological testing workflows.

• Always include positive and negative controls in each run.

• Use DNAready Lysis Buffer for efficient DNA extraction.

• Avoid contamination by separating PCR setup and sample preparation areas.

• Shipment at Ambient Temperature.
• Storage: -20 °C upon receipt. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q1: What is the detection target of this kit?
It specifically detects Listeria monocytogenes DNA.

Q2: Does the kit include an internal control?
Yes, it includes an Internal Amplification Control (IAC).

Q3: What fluorochromes are used in detection?
FAM for the Listeria target and HEX for the IAC.