qMAXSen™ One-Step Green RT-qPCR Kit (Low Rox™)

One-step Green RT-qPCR kit with Low Rox™ for sensitive, specific, and reproducible RNA detection and quantification

The qMAXSen™ One-Step Green RT-qPCR Kit Low Rox is a ready-to-use master mix that combines reverse transcription and quantitative PCR in a single tube. This one-step Green RT-qPCR kit reduces hands-on time, minimizes contamination risk, and delivers highly sensitive and reproducible RNA quantification.

Its optimized formulation includes a balanced reverse transcriptase, RNase inhibitor, and hot-start DNA polymerase to ensure efficient cDNA synthesis and robust amplification. With Low Rox™ reference dye included, the kit provides accurate normalization for platforms requiring reduced Rox levels, making it ideal for gene expression analysis, viral RNA detection, and biomarker validation.

Canvax™ qMAXSen™ qPCR Kits has been Nominee as “Best New Life Sciences Product of 2018” at Scientists’ Choice Awards®. 

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Price: 161.20 549.10 

SKU: E0833 Categories: ,

Detailed information:

Advantages & Features

  • One-step workflow: cDNA synthesis and qPCR in a single tube.
  • High sensitivity: accurate detection of low-copy RNA.
  • Reproducible: consistent performance across replicates.
  • Low Rox™ reference dye: for platforms requiring reduced dye concentration.
  • Easy-to-use: ready-to-use master mix reduces setup time.
  • Flexible: works in both standard and fast qPCR runs.

Specifications

Includes

Includes for 500 rxn (of 20 µl):
– 5 x 500 µL qMAXSen™ One-Step RT-qPCR (4x)
– 5 x 100 μL RT mix
– 5 x 1 mL RNase-free Water

Download documentation

Datasheet
MSDS

Applications

  • RNA quantification in gene expression studies.
  • Green dye-based one-step RT-qPCR assays.
  • Viral RNA detection in research workflows.
  • Transcriptomics and biomarker discovery.
  • High-throughput qPCR applications.

Tables & Figures

Quality Control

  • Functionally tested in RT-qPCR assays for activity, efficiency, sensitivity, and heat stability.

  • Verified nuclease-free (DNase, RNase, protease not detected).

Advice

  • Thaw components on ice and mix gently before use.

  • Avoid repeated freeze-thaw cycles.

  • Always include positive and negative controls in assays.

Storage, Shipping & Guarantee

  • Shipment at Gel Pack.
  • Storage: Upon receipt of the kit, immediately store the components at -20 °C in a freezer without a defrost cycle. It is recommended to avoid repeated freezing and thawing.

Citations

Ruiz, Leticia, et al. “Resistance to Cucumber Green Mottle Mosaic Virus in Cucumis melo.” Plants 10.6 (2021): 1077.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

FAQs

Q: What is the difference between High Rox™ and Low Rox™ kits?
A: The Low Rox™ version is formulated for instruments requiring reduced Rox dye levels.

Q: Is it suitable for viral RNA research?
A: Yes, the kit is optimized for sensitive viral RNA detection and quantification.

Q: Can it be used in fast qPCR runs?
A: Yes, the formulation supports both standard and fast protocols.

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