qMAXSen™ One-Step Probe RT-qPCR Kit (Low Rox™)

Name: qMAXSen™ One-Step Probe RT-qPCR Kit (Low Rox™)
SKU: E0853
Availability: InStock

For Greater Specificity, Sensitivity & Yields in cDNA Synthesis and qPCR in a single tube

The qMAXSen™ One-Step Probe RT-qPCR Kit (Low Rox™) is a ready-to-use master mix for probe-based real-time RT-qPCR, integrating reverse transcription and qPCR in a single step. This one-step RT-qPCR kit streamlines workflows by reducing pipetting errors, saving time, and minimizing contamination risk.

Thanks to its optimized enzyme mix—combining a reverse transcriptase, RNase inhibitor, and hot-start DNA polymerase—this qMAXSen One-Step Probe RT-qPCR Kit Low Rox delivers high sensitivity for low-copy RNA detection, excellent linearity, and superior reproducibility across a wide range of gene expression and viral RNA studies.

With Low Rox™ reference dye included, the kit provides precise normalization for platforms requiring reduced Rox levels, ensuring accurate quantification and reliable results in gene expression and biomarker analysis.

Canvax™ qMAXSen™ qPCR Kits has been Nominee as “Best New Life Sciences Product of 2018” at Scientists’ Choice Awards®.

Have questions? Contact us

Price: 161.20 € – 549.10 €

size

100 rxn

500 rxn

SKU: E0853 Categories: qPCR, One-Step qPCR MasterMixes

Detailed information:

Advantages & Features

One-step reaction: RT and qPCR in a single tube.
High sensitivity: detects low-copy RNA targets with accuracy.
Specific & reproducible: optimized for probe-based assays.
Low Rox™ reference dye: ensures normalization in compatible platforms.
Convenient: reduces handling time and risk of contamination.
Versatile: suitable for viral RNA detection, gene expression, and biomarker studies.

Specifications

Includes

Includes for 500 rxn (of 20 µl):
– 5 x 500 µL qMAXSen™ One-Step RT-qPCR (4x)
– 5 x 100 μL RT mix
– 5 x 1 mL RNase-free Water

Download documentation

Datasheet

MSDS

Applications

Probe-based one-step RT-qPCR assays.
RNA quantification and gene expression analysis.
Viral RNA detection and research.
Biomarker validation.
High-throughput qPCR applications.

Tables & Figures

Quality Control

Functionally tested in probe-based RT-qPCR for activity, processivity, sensitivity, and heat stability.
Certified free from DNase, RNase, and protease contamination.

Advice

Thaw reagents on ice and mix gently before use.
Avoid repeated freeze-thaw cycles.
Include negative and positive controls in each run.

Storage, Shipping & Guarantee

Shipment at Gel Pack.
Storage: Upon receipt of the kit, immediately store the components at -20 °C in a freezer without a defrost cycle. It is recommended to avoid repeated freezing and thawing.

Citations

Porcellato, I., Modesto, P., Cappelli, K., Varello, K., Peletto, S., Brachelente, C., … & Razzuoli, E. (2020). Equus caballus papillomavirus type 2 (EcPV2) in co-occurring vulvar and gastric lesions of a pony. Research in Veterinary Science.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

Synonym(s)

One-step probe RT-qPCR kit, RT-qPCR probe master mix, One-step real-time RT-PCR kit, Probe-based RT-qPCR mix, qPCR probe detection kit

Also known as:

Spanish: Kit RT-qPCR con sonda en un paso, Master mix RT-PCR en tiempo real con sonda, Kit RT-qPCR one-step
French: Kit RT-qPCR à sonde en une étape, Master mix RT-PCR temps réel à sonde, Kit RT-qPCR one-step
German: One-Step RT-qPCR Kit mit Sonde, Echtzeit RT-PCR Mastermix mit Sonde, RT-qPCR Kit
Italian: Kit RT-qPCR one-step con sonda, Master mix RT-PCR in tempo reale con sonda, Kit RT-qPCR

FAQs

Q: What is the difference between High Rox™ and Low Rox™ kits?
A: The Low Rox™ kit is formulated for instruments that require reduced levels of Rox reference dye.

Q: Can it detect viral RNA?
A: Yes, the kit is optimized for sensitive viral RNA detection and quantification.

Q: Is it compatible with fast cycling protocols?
A: Yes, the formulation supports both standard and fast RT-qPCR runs.

Q: Can this mix be used for standard PCR applications?
A: It can be used for endpoint PCR, but it is optimized for qPCR and may yield lower performance compared to dedicated PCR mixes.