Green qPCR MasterMix – Canvax high-sensitivity quantitative PCR mix

qMAXSen™ Green qPCR MasterMix (High ROX™)

Name: qMAXSen™ Green qPCR MasterMix (High ROX™)
SKU: E0539
Price: 209.00 EUR
Availability: InStock

High-sensitivity 2× master mix for qPCR with High ROX reference dye compatibility.

qMAXSen™ Green qPCR MasterMix (High ROX) from Canvax™ is a ready-to-use, 2× concentrated reaction mix optimized for precise and reproducible quantitative PCR (qPCR) using intercalating dye detection. This high-performance master mix contains a proprietary DNA polymerase, dNTPs, MgCl₂, buffer components, and an optimized concentration of ROX reference dye, ensuring excellent sensitivity, dynamic range, and reproducibility across multiple qPCR platforms.

The advanced formulation provides superior fluorescence signal, minimal background, and consistent amplification of low-copy targets, making it ideal for gene expression profiling, quantification, and genotyping assays.

Canvax™ qMAXSen™ qPCR Kits has been Nominee as “Best New Life Sciences Product of 2018” at Scientists’ Choice Awards®.

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500 rxn

Price: 209.00 €

SKU: E0539 Categories: qPCR, qPCR MasterMixes

Detailed information:

Advantages & Features

Exceptional sensitivity: Detects low-copy targets with high signal-to-noise ratio.
High ROX normalization: Compatible with qPCR instruments requiring High ROX reference dye.
Ready-to-use 2× formulation: Minimizes pipetting errors and ensures reproducible results.
Fast and robust performance: Optimized for short cycling protocols without loss of efficiency.
Wide dynamic range: Accurate quantification across several orders of magnitude.
Excellent stability: Maintains full activity after multiple freeze–thaw cycles.

Specifications

Taq Source: An E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus.

Includes

Includes for 500 reactions:
– 4 x 1.25 mL qMAXSen™ Green qPCR MasterMix 2x (High ROX)
The MasterMix includes 2x mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, Reference dye & Proprietary Buffer Components.

Download documentation

Datasheet

MSDS

Instruments Compatibility

Applications

Gene expression analysis.
DNA quantification and copy number determination.
Genotyping and SNP detection.
High-throughput screening assays.
qPCR assay development and validation.

Tables & Figures

Quality Control

Each lot is tested for amplification efficiency, sensitivity, reproducibility, and absence of nucleases.

Advice

Mix gently before use; avoid vortexing.
Always include a no-template control (NTC) to monitor contamination.
For best performance, use cDNA or purified DNA templates free of inhibitors.
Use recommended annealing temperatures for specific primer sets.

Storage, Shipping & Guarantee

Shipment at Blue Ice /Gel Pack.
Storage: -20 °C upon receipt. ROX™ reference dye is sensitive to exposure to light. Avoid repeated freezing and thawing.

Citations

Janssen, D., González-Miras, E., & Rodríguez, E. (2023). Tuta absoluta-Specific DNA in Domestic and Synanthropic Vertebrate Insectivore Feces. Insects, 14(8), 673.
Togashi, K., Hatakeyama, S., Yoneyama, T., Hamaya, T., Narita, T., Fujita, N., … & Ohyama, C. (2022). Effect of active anticancer therapy on serologic response to SARS‐CoV‐2 BNT162b2 vaccine in patients with urothelial and renal cell carcinoma. International Journal of Urology.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

FAQs

Q1: What is the difference between High ROX and Low ROX formulations?
The High ROX version contains a higher concentration of passive reference dye for normalization on instruments such as Applied Biosystems 7500 or QuantStudio platforms.

Q2: Can the mix be used with SYBR® Green assays?
Yes. It is fully compatible with intercalating dye–based qPCR detection systems.

Q3: Does QmaxSen™ Green qPCR MasterMix require additional MgCl₂?
No. The Mg²⁺ concentration is optimized for robust amplification across a wide range of templates.