qMAXSen™ Probe qPCR Mastermix (Low ROX™)

Includes for 500 reactions:
– 4 x 1.25 mL qMAXSen™ Probe qPCR Mastermix (Low ROX™)
The MasterMix includes 2x mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, Reference dye & Proprietary Buffer Components.

• Probe-based real-time PCR (qPCR).

• Gene expression quantification.

• Pathogen detection assays.

• SNP genotyping and mutation analysis.

• Copy number and viral load quantification.

• Each lot is tested for amplification efficiency, reproducibility, and absence of DNase, RNase, and protease activity to ensure reliable qPCR performance.

• Instrument Compatibility:

– Applied Biosystems: ABI 7500 and ABI 7500 Fast, ABI ViiA7.
– Stratagene(Agilent): Mx3000™, Mx3005P™, and Mx4000™, Mx4000R.
– Other instruments that do not require the use of a passive reference.

• Use 10–50 ng of DNA or 1–100 ng of cDNA per reaction.

• Mix gently before use; avoid vortexing.

• Optimize primer and probe concentrations for best results.

ROX™ is a trademark of Applera Corporation.
TaqMan® is a registered trademark of Roche Molecular Systems, Inc.
Mx3000™, Mx3005P™, and Mx4000™, Mx4000R™ are a registered trademarks of Stratagene Inc.
ABI 7500™ and ABI 7500 Fast™, ABI ViiA7™ are a registered trademarks of Applied Biosystems

Products may be covered by pending or issued patents or may have certain limitations.

• Shipment at Blue Ice /Gel Pack.
• Storage: -20 °C upon receipt. ROX™ reference dye is sensitive to exposure to light. Avoid repeated freezing and thawing.

• Mecocci, S., Capomaccio, S., Porcellato, I., Dell’Anno, F., Ratto, R., Mechelli, L., … & Cappelli, K. (2025). Deciphering the transcription regulation of bovine papillomavirus (BPV)-associated equine sarcoids through OMIC integrated approach.
• Düzgün, Ç., Dede, S., Karakuş, E., Adaş, M., & Bilen, Ö. (2022). Biochemical analysis of microbiotas obtained from healthy, prediabetic, type 2 diabetes, and obese individuals. Turkish Journal of Biochemistry.
• Gómez-Melero, S. (2022). Desarrollo de anticuerpos terapéuticos contra el receptor CCR6 humano.
• Armando, F., Godizzi, F., Razzuoli, E., Leonardi, F., Angelone, M., Corradi, A., … & Passeri, B. (2020). Epithelial to Mesenchymal Transition (EMT) in a Laryngeal Squamous Cell Carcinoma of a Horse: Future Perspectives. Animals, 10(12), 2318.
• Porcellato, I., Modesto, P., Cappelli, K., Varello, K., Peletto, S., Brachelente, C., … & Razzuoli, E. (2020). Equus caballus papillomavirus type 2 (EcPV2) in co-occurring vulvar and gastric lesions of a pony. Research in Veterinary Science.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q1: What type of probes are compatible?
It supports TaqMan® and other hydrolysis probes for accurate quantification.

Q2: What instruments require Low ROX?
Applied Biosystems® 7500 and similar platforms use low ROX normalization.

Q3: Can it be used for multiplex qPCR?
Yes. The optimized formulation supports multiplex amplification with high efficiency.