SRB Cytotoxicity Assay Kit

– 0.4 g SRB Dye
– 100 mL Fixative Reagent
– 100 mL Dye Wash Solution (10x)
– 200 mL SRB Solubization Buffer

• Cytotoxicity detection.

• Cell viability assays.

• Cell proliferation studies.

• High-throughput screening.

Each batch is tested for consistency in SRB binding and reproducibility in cytotoxicity detection, ensuring linearity and low background.

• Handle reagents with care; some are carcinogenic or corrosive.

• Work in a fume hood with protective equipment (gloves, mask, goggles).

• Protect the product from light.

• Shipped in: Ambient Temperature.
• Storage: Room Temperature.
• Recommendations: Protect the product from light.

• Kılınçarslan, M. B. (2025). MCL-1 İnhibitörü AZD5991 ile Genisteinin Kombinasyon Terapisinin MCF-7 Meme Kanseri Hücrelerinde Sinerjik Etkisinin Araştırılması (Investigation of the synergistic effect of combined therapy with MCL-1 inhibitor AZD5991 and genistein in MCF-7 breast cancer cells) Master’s Thesis, Denizli, Turkey.
• Macchia, M. L., Ricci, M., Boccalon, M., Baranyai, Z., Ghezzi, B., Audrito, V., & Botta, M. (2026). Secondary amide derivatives of Fe (III)-CDTA: Impact of ligand substitution on relaxivity, stability, and kinetic inertness. Journal of Inorganic Biochemistry, 278, 113262.
• Gnocchi, D., Kapoor, S., Nitti, P., Cavalluzzi, M. M., Lentini, G., Denora, N., … & Mazzocca, A. (2019). Novel lysophosphatidic acid receptor 6 antagonists inhibit hepatocellular carcinoma growth through affecting mitochondrial function. Journal of Molecular Medicine, 1-13.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

SRB cytotoxicity assay kit, Sulforhodamine B assay kit, Cell viability assay SRB, SRB cell proliferation assay, Colorimetric cytotoxicity assay

Also known as:

• Spanish: Kit de citotoxicidad SRB, Ensayo con sulforodamina B, Ensayo de viabilidad celular SRB
• French: Kit de cytotoxicité SRB, Test sulforhodamine B, Essai de viabilité cellulaire SRB
• German: SRB-Zytotoxizitätskit, Sulforhodamin-B-Assay, Zellviabilitätsassay SRB
• Italian: Kit citotossicità SRB, Saggio sulforodamina B, Saggio vitalità cellulare SRB

Q1: What wavelength is used for SRB detection?
OD is measured at 540 nm with a reference at 690 nm.

Q2: Can this kit be used for high-throughput applications?
Yes, it is validated for high-throughput cytotoxicity and proliferation assays.

Q3: Is the end-point stable?
Yes, SRB staining provides a stable end-point, allowing delayed analysis.