Kanamycin Sulfate
Canvax Kanamycin Sulfate, molecular biology grade aminoglycoside antibiotic for bacterial selection.
Kanamycin Sulfate is a high-purity, molecular biology-grade aminoglycoside antibiotic derived from Streptomyces kanamyceticus. It possesses a broad antibacterial spectrum including many Gram-positive, Gram-negative bacteria and mycobacteria. This reagent is designed for in vitro research use, particularly for selection of bacterial, fungal or mammalian cells carrying kanamycin resistance genes such as kanMX or NPT II, and for maintaining sterile cell culture systems by preventing bacterial contamination.
The mechanism of action is via binding to the 70S ribosomal subunits, leading to miscoding and inhibition of protein synthesis. Canvax Kanamycin Sulfate ensures lot-to-lot consistency, high activity, and low impurity levels.
For Research Use Only (RUO). Not for use in diagnostic procedures, human or veterinary applications.
Have questions? Contact us
Price: 67.50 €
Detailed information:
Advantages & Features
- ≥750 units/mg potency ensures strong selective pressure and efficient cell selection.
- White to off-white crystalline powder; high purity, low loss on drying (<2 %).
- Reliable lot-to-lot consistency via strict quality control (COA / MSDS available).
- Compatible with bacterial, fungal, and mammalian cell culture applications; ideal for selection of transformed cells.
- Water soluble; stable under recommended storage; shipped with gel pack to protect quality.
Specifications
- CAS Number: 25389-94-0
- Chemical Formula: C18H36N4O11.H2O4S
- Molecular Weight: 582.58 g/mol
- Appearance: White to off-white crystalline powder
- Loss on drying: <2%
- Potency: > 750 units/mg
Includes
5 g of Kanamycin Sulfate (Molecular Biology Grade)
Download documentation
Datasheet MSDSApplications
- Selection of kanMX, NPT II / Neo or other kanamycin resistance systems in E. coli, yeast, fungi, or mammalian cells.
- Prevention of bacterial contamination in cell and tissue culture.
- Use in cloning, transformation assays, and antibiotic selection plates or liquid media.
- Use in research involving Mycobacteria or Gram-positive/-negative bacteria sensitive to kanamycin.
Tables & Figures
Quality Control
- Strict quality controls in every manufacturing step to guarantee the highest quality.
Advice
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Prepare stock solutions in sterile water; typical working concentrations vary depending on organism: e.g. 50-100 µg/mL for bacteria, 200-400 µg/mL for mammalian selection
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Filter sterilize (0.2 µm) when needed.
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Avoid repeated freeze-thaw cycles; prepare aliquots of stock solutions for long-term storage.
Storage, Shipping & Guarantee
- Shipped in: Shipped with gel packs
- Storage: -20 °C (NON Frost-Free Freezer)
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website. Avoid inhalation, ingestion, skin or eye contact. Use appropriate PPE: gloves, lab coat, eye protection.In case of contact: rinse thoroughly with water; seek medical advice. Dispose of waste in accordance with institutional, local, and national regulations.
Customers Review
“We saw excellent selection results with low background using Canvax Kanamycin at 100 µg/mL in E. coli” — University of Seville
“Reliable potency across batches; highly consistent results.” — French Company
Synonym(s)
Kanamycin sulfate antibiotic, Kanamycin antibiotic, Aminoglycoside antibiotic kanamycin, Kanamycin reagent, Antibiotic for selection
Also known as:
- Spanish: Sulfato de kanamicina, Antibiótico kanamicina, Antibiótico aminoglucósido
- French: Sulfate de kanamycine, Antibiotique kanamycine, Antibiotique aminoglycoside
- German: Kanamycinsulfat, Kanamycin-Antibiotikum, Aminoglykosid-Antibiotikum
- Italian: Solfato di kanamicina, Antibiotico kanamicina, Antibiotico aminoglicoside
FAQs
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What is the working concentration for bacteria?
Typically 50–100 µg/mL; check strain sensitivity. -
Can it be used for mammalian cell selection?
Yes, but higher concentrations (200–400 µg/mL) may be required. -
Is it soluble in water?
Yes, easily soluble (e.g., 50 mg/mL). Poorly soluble in organic solvents. -
Does it affect eukaryotic cells without resistance genes?
Yes, it can be toxic if resistance gene is absent.
Takumi Sato –
We mainly use this antibiotic for bacterial selection during plasmid propagation. Solubility and preparation were convenient for our routine media batches, and we did not observe variability between experiments. Delivery and packaging were also good for frequent laboratory use.
Hannah Wilson –
We use kanamycin sulfate regularly for bacterial selection during cloning and plasmid maintenance experiments. Preparation is simple and it fits well into standard microbiology workflows. It has been practical for routine culture work in E. coli systems.