Chromatography Buffer (5x)

Sterile ready-to-use NaCl + Sodium Phosphate + EDTA buffer solution.

Chromatography Buffer (5×) is a sterile, ready-to-use buffer solution composed of 5 M Sodium Chloride, 50 mM Sodium Phosphate, and 2 mM EDTA. This balanced formulation provides optimal ionic strength, pH stabilization, and protection against metal ion–dependent degradation, ensuring consistent results in molecular biology and biochemical applications.

The buffer is ideal for protein purification, DNA and RNA preservation, enzymatic assays, and general biochemical workflows. It maintains pH stability and ionic strength during chromatographic separations, supporting reliable elution profiles and minimizing non-specific binding. Its sterile manufacturing process guarantees contamination-free performance, enabling reproducibility in sensitive and high-precision experiments.

Produced in an ISO 13485 and GMP-certified facility under ISO 9001 guidelines, this buffer ensures nuclease-free quality, batch-to-batch consistency, and dependable performance for a wide range of ion exchange and affinity chromatography applications.

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(1 customer review)

Price: 70.80 283.20 

SKU: BR0371 Categories: ,

Detailed information:

Advantages & Features

  • Sterile formulation: prevents contamination in molecular workflows.

  • Optimized composition: combines NaCl, phosphate buffer, and EDTA.

  • Ready-to-use: eliminates preparation time and variability.

  • Certified quality: produced in a ISO 13485:2016 and GMP certified plant, under ISO 9001:2016.

  • Versatile: suitable for nucleic acids, proteins, and enzymatic studies.

Specifications

  • Format: Liquid, Ready-to-use solution
  • Composition: Sodium Chloride (28-30%); Sodium Phosphate (Monobasic) (< 1%); Sodium Phosphate (Dibasic) (<1%); EDTA (<1%) and WFI Water up to rest.
  • Sterilization: purified using a 0.22 µm filtration process [aseptically filled]

Includes

  • Includes for 1L: 1,000 mL solution in PET Bottle
  • Includes for 5L: 5L solution in 5L graduated square containers with moulded hand grip.

Download documentation

Datasheet
MSDS

Applications

  • DNA and RNA stabilization.

  • Protein preservation and assays.

  • Enzymatic reactions.

  • General molecular biology workflows.

Tables & Figures

Quality Control

Manufactured in an ISO 13485:2016 and GMP-certified facility, located in Boecillo, Valladolid (Spain), under ISO9001:2016 procedures to ensure the highest quality standards. Our processes include:

    • Batch-to-batch consistency.
    • Facilities audited regularly Quality audits.
    • Lot specific CoAs and QA review previously to release a product.
    • Material traceability, documented procedures and documented employee training.
    • Equipment maintenance and monitoring records.

Request a Certificate of Analysis (CoA)

Advice

  • Use directly without further dilution.

  • Keep container tightly closed after opening.

  • Aliquot if frequent use is required to minimize contamination risk.

Storage, Shipping & Guarantee

  • Shipping: Ambient Temperature.
  • Storage: Room Temperature or at 4 ºC.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

Synonym(s)

Chromatography buffer concentrate, Buffer solution for purification, Protein purification buffer, Column buffer solution, Concentrated buffer 5x

Also known as:

  • Spanish: Buffer de cromatografía concentrado, Solución tampón para purificación, Buffer 5x
  • French: Tampon de chromatographie concentré, Solution tampon pour purification, Tampon 5x
  • German: Chromatographiepuffer Konzentrat, Pufferlösung zur Reinigung, Puffer 5x
  • Italian: Tampone di cromatografia concentrato, Soluzione tampone per purificazione, Tampone 5x

FAQs

Q1: Is this buffer sterile?
Yes, it is sterile and tested nuclease- and protease-free.

Q2: Can it be used with nucleic acids and proteins?
Yes, the formulation is optimized for both DNA/RNA preservation and protein stabilization.

Q3: Which standards apply to production?
It is produced in an ISO 13485:2016 and GMP-certified facility under ISO 9001:2016 standards.

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    1 review for Chromatography Buffer (5x)

    1. Ahmed Hassan

      We incorporated this buffer into our protein purification workflow. The concentrated format allows us flexible preparation of working solutions depending on our experiment scale. It simplifies our preparation and reduces preparation time in routine runs. The product fits well within standard our purification setups.

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