FastPANGEA™ Long PCR DNA Polymerase
High-fidelity polymerase optimized for amplification of long and GC-rich DNA templates.
FastPANGEA™ Long PCR DNA Polymerase is a high-performance enzyme designed for amplification of long DNA fragments. It combines strong processivity with high fidelity, ensuring accurate and reproducible results.
This polymerase is ideal for long-range PCR and complex DNA templates, providing robust yields and excellent efficiency across various reaction conditions.
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Price: 31.32 €
Detailed information:
Advantages & Features
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High processivity: PCR amplification of fragments up to 15 kb such as 5-15 kb of genomic DNA.
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Proofreading activity: 3′→5′ exonuclease function ensures high fidelity.
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Optimized buffer system: Enhances performance with GC-rich and long templates.
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Exceptional accuracy: Reduces base misincorporation rates in long PCR.
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Robust amplification: Delivers reproducible results even from low-copy targets.
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Versatile compatibility: Suitable for genomic, plasmid, or cDNA templates.
Specifications
Unit definition:
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP’s into acid-insoluble form in 30 minutes at 75 °C under assay conditions: 25 mM TAPS-HCl, pH 9.0 (at 25 °C), 100 mM KCl, 1.5 mM MgCl2, 1 mM Beta-mercaptoethanol, 200 μM each dNTP and 10 μg activated calf thymus DNA in 50 μl.
Includes
– 100 U FastPANGEA™ Long PCR DNA Polymerase
– 500 μL Buffer PA (5x)
– 250 μL MgCl2 (50 mM)
– 60 μL DMSO (100%)
Download documentation
Datasheet MSDSApplications
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Long-range PCR and cloning.
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Amplification of GC-rich templates.
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Sequencing and mutation analysis.
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Template generation for molecular cloning.
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High-fidelity DNA synthesis for downstream studies.
Tables & Figures
Quality Control
- Each batch is tested for purity, fidelity, activity, and absence of DNase and RNase contamination, ensuring reproducible amplification results.
Advice
- Highly recommended for cloning with pSpark® DNA cloning vectors.
- Optimize Mg²⁺ concentration and extension time for long templates.
- Use freshly prepared DNA to maximize yield and accuracy.
- Avoid excessive cycling to prevent nonspecific amplification.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What is the maximum fragment length amplified by FastPANGEA™ Long PCR DNA Polymerase?
Up to 15kb from genomic DNA templates.
Q2: Can this enzyme be used for GC-rich targets?
Yes. The optimized buffer system ensures efficient amplification of GC-rich and repetitive sequences.
Q3: Does the enzyme have proofreading activity?
Yes. It includes 3′→5′ exonuclease activity for high fidelity and error correction.
