Tris-Glycine Buffer (pH 8.3)
High Quality Molecular Biology Grade Buffer for a Reliable & Convenient use in a wide range of Applications
Tris-Glycine Buffer (TG) is a high quality and convenient running buffer in native (non-denaturing) homogeneous and gradient poly-acrylamide gel electrophoresis (PAGE) of proteins. Tris-glycine gels resolve proteins by size. However, very small proteins and peptides do not resolve well due to interference from the glycine/pH discontinuity front.
It is also used to make Tris-glycine/20% methanol Western transfer buffer, which is the most frequently used protein transfer buffer for wet blot transfers.
GMP-grade is also available. Learn more.