6X B/Loading Buffer
For visual Tracking of DNA Migration during Electrophoresis
6X B/Loading Buffer is used as a loading dye for visual tracking of DNA migration during electrophoresis. It incorporates Bromophenol blue. Bromophenol blue migrates fast in the agarose gel and corresponds to the migration of a 300 – 500 bp long DNA fragment in a 1% agarose gel.
EDTA is included in the solution to protect sample from nuclease degradation. Glycerol is added to provide high density to the solution. Due to its high density, sample settles at the bottom of the well. It also helps DNA samples to be confined in the well without diffusing out from it.