HotBegan™ Hot Start Green-Taq MasterMix
For a Specific, Highly Efficient & Reliable Amplifications of DNA up 160 fg, with visual Tracking of DNA Migration
HotBegan™ Hot Start Green-Taq Master Mix (2x) is an optimized ready-to-use solution containing HotBegan™ Hot Start Taq DNA Polymerase, dNTPs, MgCl2 and Stabilizers. It is inactive at room temperature and only requires addition of template, primers, and water.
HotBegan™ Hot Start Taq DNA Polymerase is a Taq DNA Polymerase bound to a proprietary antibody that blocks Polymerase activity until denaturation step occurs. The heat labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and nonspecific products.
MasterMix also contains an Agarose Loading Buffer including a including two tracking dyes (blue and yellow dye) for visual tracking of DNA migration and a dense compound to facilitate the drop-down of the samples into the well agarose gels. The blue dye (migrates with 3 to 5 kb DNA fragments in 1% agarose gel) and the yellow dye (migrates faster than 10 bp DNA fragments in 1% agarose gel).
Like the Taq polymerase, the enzyme has 5’→3’ Polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of enzyme activities are detectable.