T7 RNA Polymerase (50,000 U/ml)

High Quality & Proven Performance in RNA In Vitro Transcription Reactions

T7 RNA Polymerase is a recombinant protein from the bacteriophage T7 that is routinely used to produce RNA sequences from DNA templates. The protein shows highly stringent specificity for its own promoter sequence that besides its consistent yields makes it suitable for producing large amount of a designed RNA. This recombinant version is purified from E. coli.

GMP-grade is also available. Learn more.

Price: 62.00

SKU: EZ0025 Categories: ,

Detailed information:

Advantages & Features
  • Isolated form a recombinant source.
  • High-Purity: ≥ 95% determined by SDS.
  • Extensivelly quality controlled: Endo/exonucleases, non-specific DNAse and RNAses free, Purity and Promoter Specificity.
  • GMP-grade available.
Specifications

Protein information
Predicted MW: 98,855 kDa
Purity: ≥ 95% as determined by SDS-polyacrylamide gels with Coomassie® blue staining.
Buffer: 1X Reaction Buffer: 40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine (pH 7.9 @ 25°C).
Biological activity: One unit is defined as the amount of enzyme required to generate 1 nmol RNA in 1 hour at 37°C determined by a fluorimetric method.

Gene Information
Synonyms: T7RNAP
Official Symbol: T7p07
Specie: Escherichia phage T7
GenBank accession number: NC_001604.1
NCBI Protein RefSeq: NP_041960.1
Protein Family: Family of single-subunit RNAPs that includes the phage RNAPs (T3,K11, SP6, N4, and others) as well as the mitochondrial RNAPs.

Includes

– 5,000 U T7 RNA Polymerase (50 U/µL)
– T7 RNA Polymerase Reaction Buffer

Download documentation
Datasheet
Applications
  • Synthesis of mRNA, RNA standard template for RT-PCR, RNA probes for hybridization.
  • Preparation of RNA vaccines.
  • Studies of RNA secondary structure and RNA-protein interactions, RNA splicing.
Tables & Figures

Quality Control

Quality Tests for Research Use Only version includes: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.

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Advice
Storage, Shipping & Guarantee
  • Shipped in: Gel Pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

To be informed about our Safety Staments for GMP Products, please contact us.

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