Inorganic Pyrophosphatase (100 U/ml)

High Quality & Proven Performance in RNA In Vitro Transcription Reactions

Inorganic Pyrophosphatase, Recombinant, can be found in many different organisms controlling the cellular concentration of Inorganic Pyrophosphate (PPi) catalyzing its hydrolisis to form orthophophate. This recombinant version is purified from an E. coli strain overexpressing an E. coli gene. This protein is widely used in RNA In Vitro Transcription (IVT) to reduce the inhibitory effects of PPi.

 

GMP-grade is also available. Learn more.

Price: 236.00

SKU: EZ0035 Category:

Detailed information:

Advantages & Features
  • Optimized for enhancing DNA replication avoiding the inhibitory effect of PPi and subsequently increasing RNA yield in the In Vitro Transcription reactions.
  • Isolated form a recombinant source.
  • Extensivelly quality controlled: Endo/exonucleases, and RNAses free.
  • High-Purity: ≥ 95% determined by SDS.
  • Flexible Quality Systems that covers from RUO to cGMP.
Specifications

Protein information
Predicted MW: Homohexamer with 19,71 kDa subunits
Purity: ≥ 95% by SDS-PAGE
Buffer: 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2, 2 mM PPi
Storage: 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH=8
Biological activity: One unit is the amount of enzyme that will generate 1 µmol ofphosphate per minute from PPi under standard reaction conditions (10 minutes reaction at 25°C) in reaction buffer.

Gene Information
Synonyms: PPAse
Official Symbol: E-PPAse
Specie: Escherichia coli
GenBank accession number: NC_000913 (4449125-4449652)
NCBI Protein RefSeq: NP_418647
Protein Family: Bacterial and archae inorganic pyrophosphatases, hydrolases

Includes

– 50 U Pyrophosphatase, Inorganic, from E. coli (0,1 U/µL)

Download documentation
Datasheet
MSDS
Applications
  • RNA in vitro transcription (IVT) to reduce the inhibitory effects of PPi.
  • mRNA-based Vaccines.
  • RNA Labeling.
  • Enhancement of DNA replication.
Tables & Figures

Quality Control

Quality Tests for Research Use Only version includes: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.

Request your CoA here.

Advice

Avoid exposure to constant temperature changes.

Storage, Shipping & Guarantee
  • Shipped in: Gel Pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements

This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

To be informed about our Safety Staments for GMP Products, please contact us.

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