CVX™ T7 ARCA mRNA Synthesis Kit
For Easy, Scalable & High-Quality in vitro ARCA-capped (m)RNA Synthesis by T7 RNA Polymerase
CVX™ T7 ARCA mRNA Synthesis Kit is designed for in vitro ARCA-capped (m)RNA Synthesis by T7 RNA Polymerase. The cap structure is added to the mRNA by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA). ARCA is incorporated into mRNA exclusively in the correct orientation, generating capped mRNA that is more efficiently translated.
By using a DNA template encoding a poly(A) tail, the CVX™ T7 ARCA mRNA Kit can be used to synthesize capped and tailed mRNAs.
mRNAs synthesized with the CVX™ T7 ARCA mRNA Kit can subsequently be used for cell transfection, microinjection, in vitro translation and RNA vaccines. The kit contains sufficient reagents for 50 reactions of 20 μl each. A 20 μl reaction yields about 50 μg RNA with 1 μg of template added (1.5 kb RNA transcript). The T7 RNA Polymerase Enzyme mix includes Ribonuclease Inhibitor Protein to protect newly transcribed RNA from degradation by RNases.