PRImeDETECT™ STEC Detection Kit
For a Fast & Reliable Amplification and Detection of specific DNA fragments from Sof the virulence-associated genes stx1, stx2 and eae by the real-time PCR
PRImeDETECT™ STEC Detection Kit is an ideal tool for an specific, rapid, and reliable Amplification and Detection of the virulence-associated genes stx1, stx2 and eae by the real-time PCR method (Multiplex PCR).
The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, MgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors.
Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The Probe for the detection of target gene is labelled with the FAM (sxt1), HEX (stx2) and ROX (eae) fluorochrome, whereas the probe for the detection of IAC is labelled with the CY5 fluorochrome.
In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.