PBS (pH 7.2)
High Quality and sterile solution for Research and Further Manufacturing applications
PBS (pH 7.2) is a phosphate-buffered saline solution used in cell culture and molecular biology applications. It maintains physiological osmolarity and pH, supporting cell integrity during washing or dilution steps.
This ready-to-use buffer is sterile and stable, ensuring reproducible performance in biochemical and cell-based assays.
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Price: 22.75 € – 1,332.10 €
Detailed information:
Advantages & Features
- Reliable: Manufactured in an ISO 13485:2016 and GMP-certified facility, ensuring consistent quality across batches.
- Versatile: Designed for a wide range of applications in both Manufacturing and Research.
- High Purity: due additional steps to eliminate impurities, offering low levels of endotoxins, and microbial contaminants.
- Sterile: Filtered through a 0.22-micron filter for guaranteed sterility.
- Quality Guaranteed: CoA for each lot of products manufactured supplied upon request.
- Secure Supply Chain: Strategic location and extensive expertise in regulatory compliance and global shipping ensure reliable supply.
- Optimized Efficiency & Cost Reduction: Larger batch volumes streamline QC testing, while just-in-time solutions help reduce cycle times and capacity constraints.
- Expert Regulatory & Technical Support: Industry-leading expertise mitigates raw material risks and ensures consistency between batches.
Specifications
Formula:
| Component Name | CAS | MW |
| Sodium Phosphate Dibasic | 7558-79-4 | 141.96 |
| Potassium Phosphate Monobasic | 7778-77-0 | 136.09 |
| Potassium Chloride | 7447-40-7 | 74.55 |
| Sodium Chloride | 7647-14-5 | 58.44 |
| WFI Quality Water | 7732-18-5 | 18.02 |
Standards Analytics (1x)
| Test | Specificitation | Method reference |
| Appearance | Clear and Colourless liquid | Ph. Eur. 2.2.1; 2.2.2 and 2.9.20. |
| pH | 7,0 – 7,4 | Potentiometry Ph Eur 2.2.3 |
| Osmolarity | 280 – 310 mOsm/kg | Ph. Eur. 2.2.35 |
| Bioburden | <0.1 CFU/mL | Ph. Eur. 2.6.12 |
| Endotoxin testing | < 1 EU/mL | Ph. Eur. 2.6.14 |
Includes
BR0025, BR0026 and BR0027:
– 1,000 mL PBS Solution (1X, 10X or 20X) in PET bottle.
BR0028:
– 5 L PBS Solution (1X) in 5L graduated square containers with moulded hand grip.
BR0035 and BR0036:
– 20 L PBS Solution (1X or 10X) in 20L graduated square containers with moulded hand grip.
BR0037:
– 100 L PBS Solution (1X) in single-use bag with plastic drum.
Download documentation
Datasheet MSDSApplications
Designed for a wide range of Further Manufacturing and Research applications:
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- Cell culture manufacturing.
- Upstream and Downstream processes.
- Dilute substances to working concentration.
- Used to immobilize a substance, as a protein, in a solid surface.
- Immuno‐histochemical, ELISA and Western blot assays.
- Microbiological procedures.
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Tables & Figures
Quality Control
Manufactured in an ISO 13485:2016 and GMP-certified facility, located in Boecillo, Valladolid (Spain), under ISO9001:2016 procedures to ensure the highest quality standards. Our processes include:
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- Batch-to-batch consistency.
- Facilities audited regularly Quality audits.
- Lot specific CoAs and QA review previously to release a product.
- Material traceability, documented procedures and documented employee training.
- Equipment maintenance and monitoring records.
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Advice
Storage, Shipping & Guarantee
- Shipped at: Ambient temperature.
- Storage: Room temperature (15 – 25 ºC).
- Shelf life: 24 months from date of manufacturing.
Citations
- Benítez-González, A. M., Stinco, C. M., Brnčić, M., Barba, F. J., & Meléndez-Martínez, A. J. (2025). Bioactive Lipophilic Antioxidants (Carotenoids, Tocols, Retinol, and Coenzyme Q10) in Human and Animal Tissues: Development and Validation of a Rapid Extraction and Chromatographic Method for Nutrition and Health Studies. Antioxidants, 15(1), 43.
- Onorini, D., Leonard, C. A., Phillips Campbell, R., Prähauser, B., Pesch, T., Schoborg, R. V., … & Borel, N. (2023). Neisseria gonorrhoeae Coinfection during Chlamydia muridarum Genital Latency Does Not Modulate Murine Vaginal Bacterial Shedding. Microbiology Spectrum, e04500-22.
- Fernandes, L. M. (2022). Análise da expressão de genes biomarcadores de bem-estar por hibridização in situ de ARN em paralarvas de cultivo de Octopus vulgaris (Cuvier, 1797) (Doctoral dissertation).
- Murillo, A., Cuenca, R., Serrano, E., Marga, G., Ahmed, A., Cervantes, S., … & Pastor, J. (2020). Leptospira Detection in Cats in Spain by Serology and Molecular Techniques. International Journal of Environmental Research and Public Health, 17(5), 1600.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO) and Further Manufacturing. The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
Phosphate buffered saline, PBS buffer solution, Saline phosphate buffer, PBS solution pH 7.2, Isotonic buffer PBS
Also known as:
- Spanish: Tampón PBS pH 7.2, Solución salina fosfato, Buffer fosfato salino
- French: Tampon PBS pH 7.2, Solution saline phosphate, Tampon phosphate salin
- German: PBS-Puffer pH 7.2, Phosphatgepufferte Salzlösung, Isotoner Puffer
- Italian: Tampone PBS pH 7.2, Soluzione salina fosfato, Tampone fosfato salino
FAQs
3 reviews for PBS (pH 7.2)
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Frederik Jensen –
This PBS is part of our daily laboratory routine for washing cells and preparing samples. Having it supplied ready to use saves preparation time and improves consistency between users. It has become one of the reagents we always keep in stock.
Nicole Andersen –
We use PBS throughout the day for cell washing and sample handling, so having a ready-to-use solution is very convenient. The product fits seamlessly into our routine workflows and helps reduce preparation time when several experiments are running simultaneously.
Ryan Collins –
This buffer is part of our routine cell culture and washing workflows. Ready integration into existing protocols is always important for shared laboratories, and this product has been convenient for that purpose. The packaging format also works well for frequent bench use.