PI/ Cell Cycle Analysis Kit

– 2 mL Propidium Iodide (PI) Reagent
– 50 mL Phosphate Buffered Saline (10x)
– 2 mL RNase A Solution

• Cell cycle regulation studies.

• Monitoring cell cycle progression.

• Drug effect evaluation on cell growth and division.

• Cell proliferation analysis.

• Each lot is tested for fluorescence signal reproducibility, sterility, and consistent DNA staining performance.

• Protect PI reagent from light to maintain fluorescence activity.

• Handle with care; PI is a potential mutagen.

• Always use protective equipment when handling reagents.

• Shipped at: Ambient Temperature.
• Storage: Upon receipt, store RNase A solution at -20 °C. Store the PI Reagent and 10x PBS at +4 °C and protect it from light.

• Walunj, D. T., Wu, B., Olugbami, J. O., Johnston, A., Kern, R., Nelson, T. J., … & Oyelere, A. K. (2026). Repositioning Antihistamine for Cancer Therapy: Clemizole as a Template for the Design of Liver Tissue-Targeting Epigenetic-Modifying Agents. Journal of Medicinal Chemistry 69, 3, 2238–2257
• El-Wakf, A., Elgharieb, A., & Abdrabouh, A. (2025). Chamomile tea as a supplement therapy relieves thinner induced-lung injury in rats through inhibiting inflammation, oxidative stress and P53-dependant apoptotic pathways. Beni-Suef University Journal of Basic and Applied Sciences, 14(1), 127.
• Oh, M. J., Batmunkh, B., & Kim, S. H. (2025). Comparison of apoptosis induction in colon cancer cells according to anticancer drug function. Pakistan Journal of Zoology, 57.
• Khojasteh, A., Metón, I., Camino, S., Cusido, R. M., Eibl, R., & Palazon, J. (2019). In Vitro Study of the Anticancer Effects of Biotechnological Extracts of the Endangered Plant Species Satureja Khuzistanica. International journal of molecular sciences, 20(10), 2400.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Propidium iodide cell cycle kit, Cell cycle analysis assay kit, DNA content analysis kit, Flow cytometry cell cycle kit, PI staining cell cycle assay

Also known as:

• Spanish: Kit de análisis del ciclo celular con PI, Kit de tinción con ioduro de propidio, Análisis de contenido de ADN por citometría
• French: Kit d’analyse du cycle cellulaire au PI, Kit de coloration à l’iodure de propidium, Analyse du contenu en ADN
• German: Zellzyklus-Analysekit mit PI, Propidiumiodid-Färbekit, DNA-Gehaltsanalyse
• Italian: Kit analisi ciclo cellulare con PI, Kit colorazione ioduro di propidio, Analisi contenuto DNA

Q1: Why is RNase A included in the kit?
To remove RNA, ensuring PI binds selectively to DNA.

Q2: What cell number can be used for this assay?
The standard protocol recommends 2–5 × 10⁵ cells per sample. However, higher cell numbers (e.g. up to 1 × 10⁶ cells) may also be used, provided that proper resuspension and homogeneous staining are ensured.

Q3: How should cells be detached prior to staining?
Cells can be harvested using different approaches depending on the cell type, including enzymatic detachment (e.g. trypsinization) or mechanical methods such as a cell scraper.

Q4: Are variations in centrifugation conditions acceptable?
While the standard protocol specifies centrifugation at 500 × g, slight variations in centrifugation speed may be used as long as cell integrity is maintained and pellets are not disrupted during processing.