Senescence Detection Kit
Easy-to-use SA-β-Gal staining kit for reliable detection of senescent cells.
The Senescence Detection Kit (SA-β-Gal Staining) provides a simple, fast, and reliable method for detecting senescent cells based on β-galactosidase activity at pH 6.0. This histochemical assay allows direct visualization of senescent cells by blue staining under a standard light microscope.
The kit is ideal for analyzing cellular aging, drug response, and tumor biology, enabling researchers to correlate senescence with changes in cell morphology, proliferation, and viability. With ready-to-use reagents and a straightforward protocol, the Senescence Detection Kit ensures reproducibility and consistent results across different cell types.
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Price: 159.80 €
Detailed information:
Advantages & Features
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Direct visualization: blue staining of senescent cells under light microscopy.
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Reliable performance: detects β-galactosidase activity at pH 6.0.
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Convenient format: complete kit with all essential reagents.
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Reproducible results: validated across multiple cell types.
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Versatile use: applicable in drug response and aging studies.
Specifications
Includes
– 150 mg X-Gal (lyophilized)
– 60 mL PBS (10x)
– 15 mL Fixative Solution (10x)
– 15 mL Staining Solution (5x)
– 1 x 1,5 mL Staining Solution Supplement A (100x)
– 1 x 1,5 mL Staining Solution Supplement B (100x)
Download documentation
Datasheet MSDSApplications
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Detection of senescent cells.
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Cellular aging and longevity studies.
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Drug response evaluation.
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Cancer and tumor biology research.
Tables & Figures
Quality Control
- Each batch is tested using positive control senescent cells to ensure strong and specific β-galactosidase staining at pH 6.0.
Advice
Caution:
- The fixative solution contains formaldehyde and glutaraldehyde, which are toxic and corrosive solutions. Wear personal protective clothing when handling solutions and use in a fume hood.
- Staining solution supplements (A and B) contains K4[Fe(CN)6] 3H2O and K3[Fe(CN)6], which are irritants for humans and dangerous for the environment. Wear personal protective clothing (e.g., nitrile or latex gloves, lab coat and goggles) when handling solution and discard in an appropriate manner.
- Dimethylformamide is toxic and harmful. Wear personal protective clothing when handling solution. Use a fume hood. Always use a polypropylene container or glass to make and store the X‐gal. Do not use polystyrene.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage:
- X-Gal (150 mg, Lyophilized) at -20°C, protected from light.
- 10X Fixative Solution, 10X Staining Solution and 100X Staining Supplement (A and B) can be stored at +4°C. If precipitation occurs, simply warm up the solution to 37°C to solubilize the precipitates.
- Store the 10x PBS solution at Room Temperature or 4ºC.
Citations
- Fafián-Labora, J. A., Rodríguez-Navarro, J. A., & O’Loghlen, A. (2020). Small Extracellular Vesicles Have GST Activity and Ameliorate Senescence-Related Tissue Damage. Cell Metabolism.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What marker is used to detect senescent cells?
SA-β-Gal activity at pH 6.0 is the standard marker for senescence.
Q2: Which microscope is required?
Any standard light microscope is suitable for visualization.
Q3: Can this kit be used in drug screening?
Yes, it is widely applied in drug response and tumor biology studies.
