Firefly Luciferase Assay Kit
Sensitive, ready-to-use Firefly Luciferase Assay Kit for gene expression analysis.
The Firefly Luciferase Assay Kit enables highly sensitive and quantitative measurement of firefly luciferase activity in mammalian, bacterial, or yeast cell lysates. Based on bioluminescent reaction principles, it provides fast, linear, and reproducible results suitable for both high-throughput and manual assays.
This kit is ideal for gene reporter studies, promoter analysis, and cell signaling research. Its optimized reagents ensure low background noise and high signal intensity, offering outstanding precision in gene expression quantification.
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Price: 79.90 € – 402.70 €
Detailed information:
Advantages & Features
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High sensitivity: detects low levels of luciferase activity.
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Fast and reproducible: provides consistent results in less than 5 minutes.
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Ready-to-use reagents: simplify workflow and reduce assay time.
- Flexible format: compatible with multiwell plates and luminometers.
Specifications
Includes
Includes for 100 assays:
– 10 mL Luciferase Assay Substrate
– 4 mL Cell Lysis Buffer (5x)
– 10 μg Luciferase (Control)
Download documentation
Datasheet MSDSApplications
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Reporter gene assays.
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Promoter activity studies.
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Signal transduction research.
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Gene expression quantification.
Tables & Figures
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Quality Control
- Each lot is validated for sensitivity, linearity, and reproducibility across assay replicates.
Advice
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Handle reagents under dim light to preserve enzyme activity.
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Mix gently before use; avoid foam formation.
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Use freshly prepared lysates for optimal results.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C or below, protected from light. The kit is stable at –20 °C for three months and at –70 °C for up to 2 year from date of receipt. Avoid repeated freeze-thaw cycles.
Citations
- Segovia Trinidad, C. L. (2023). Refolding, purification, and characterization of human-Smad8 produced in E. coli in form of inclusion bodies.
- Segovia-Trinidad, Carla Lizbeth, et al. “Refolding, purification, and characterization of constitutive-active human-Smad8 produced as inclusion bodies in ClearColi® BL21 (DE3).” Protein Expression and Purification (2021): 105878.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
Firefly luciferase assay kit, Luciferase reporter assay, Bioluminescence luciferase assay, Luciferase activity assay, Reporter gene assay kit
Also known as:
- Spanish: Kit de ensayo de luciferasa Firefly, Ensayo de luciferasa, Sistema de genes reporteros
- French: Kit d’analyse luciférase Firefly, Test luciférase, Système de gènes rapporteurs
- German: Firefly-Luciferase-Assaykit, Luciferase-Test, Reportergen-Assaysystem
- Italian: Kit saggio luciferasi Firefly, Saggio luciferasi, Sistema gene reporter
FAQs
Q: What type of luciferase does this kit detect?
It specifically measures firefly luciferase activity.
Q: Is this kit suitable for high-throughput screening?
Yes, it is compatible with 96- and 384-well plate luminometers. White plates are recommended to maximize luminescence signal detection.
Q: What type of instrument is required to measure the signal?
A: Any luminometer or plate reader capable of detecting luminescence with high sensitivity (femtomole ATP range) can be used.
Q: Does it require additional substrates?
No, the kit includes all reagents for direct luminescence detection.
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