Firefly & Renilla Luciferase Single Tube Assay Kit

– 10 mL Cell Lysis Buffer (5x)
– 10 mL Fluc Assay Buffer
– 10 mL Rluc Assay Buffer
– 2 x 1 mg D-Luciferin, potassium salt
– 2 x 200 μg Coelenterazine (lyophilized)

• Dual-reporter gene assays.

• Transcriptional regulation studies.

• Gene expression analysis.

• High-throughput screening.

• Each batch is validated for sensitivity, linearity, and reproducibility in Firefly and Renilla luminescence detection.

• Use fresh reagents and avoid repeated freeze-thaw cycles.

• Protect assay components from light.

• Handle luciferase substrates carefully to avoid degradation.

• Shipment at Dry Ice.
• Storage: -80°C or below. Fluc and Rluc Assay Buffers are stable at -80°C for at least three months from date of receipt. Other components are stable at -20°C or below for at least three months from date of receipt. Kit components are stable to at least 5 freeze/thaw cycles.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q1: What makes this kit different from single-luciferase assays?
It allows simultaneous Firefly and Renilla detection from the same extract.

Q2: Is it suitable for high-throughput applications?
Yes, the single-tube format is optimized for HTS.

Q3: How stable are the assay reagents?
Buffers are stable at –80 °C for 3 months; lyophilized substrates at –20 °C.