ROS Detection Assay Kit
Sensitive and safe fluorescent assay kit for quantitative detection of intracellular ROS levels.
The ROS Detection Assay Kit (DCFDA / H₂DCFDA) from Canvax™ provides a simple and sensitive method to measure reactive oxygen species (ROS) in live cells. It uses DCFDA, a cell-permeable fluorogenic dye that is oxidized by ROS to a highly fluorescent compound, allowing rapid detection through fluorescence measurement. The assay enables researchers to monitor oxidative stress, cellular metabolism, or drug-induced ROS generation in real time with excellent reproducibility.
Canvax™ ROS Detection Kit is optimized for both fluorescence microplate readers and microscopy applications. It offers high sensitivity, low background, and robust performance across multiple cell types. Manufactured under ISO-certified conditions, it ensures batch-to-batch consistency and reliable quantification of intracellular ROS. This kit is ideal for toxicology, oxidative stress, and cell biology studies requiring accurate and reproducible ROS detection.
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Price: 245.50 €
Detailed information:
Advantages & Features
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Based on unique fluorescent probe H2DCFDA.
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High sensitivity: detects hydroxyl, peroxyl, and other ROS.
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Fast & easy protocol: convenient workflow.
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Safe: non-radioactive, no special handling required.
Specifications
Includes
– 5 mg Fluorescent Substrate (H2DCFDA)
– 50 mL 10X Assay Buffer
– 500 μL Positive control (H2O2, 9.7M)
– 1 mL DMSO
Download documentation
Datasheet MSDSApplications
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Measurement of intracellular ROS.
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Oxidative stress studies.
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Cell signaling & redox biology.
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Drug discovery and toxicology assays.
Tables & Figures
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Quality Control
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Validated in multiple cell lines.
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Tested for reproducibility and background-free signal.
Advice
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Protect dye from light.
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Prepare fresh working solutions.
Storage, Shipping & Guarantee
- Shipment Gel Pack.
- Storage: 4 °C upon receipt.
Citations
- Koper, F., Grzywna, D., Svobodová, L., Sedlář, A., Kobera, L., Riedel, T., … & Kasprzyk, W. P. (2025). Cross-linked poly (alkylene citrates) with l-glutathione for vascular tissue engineering: structure–property relationships and cell-type dependent response to oxidative stress. Journal of Materials Chemistry B.
- Di Lorenzo, R., Ferraro, M. G., Carrera, C., Iazzetti, F., Chinchilla, N., Maisto, M., … & Laneri, S. (2025). Valorization of Arbutus unedo L. Pomace: Exploring the Recovery of Bioactive Phenolic Compounds from Distillation By-Products. Antioxidants, 14(3), 278.
- Martínez Cid, P. (2024). Innovación en modelos in vitro: Resistencia a la ferroptosis en células de cáncer de ovario epitelial.
- López Calvo, I. (2023). Estudio del papel de la ferroptosis en la resistencia al tratamiento quimioterapéutico en Cáncer de Ovario.
- Parn, K. W. (2023). Safety and efficacy of oral epigallocatechin gallate in attenuating hypertension in spontaneously hypertensive rats via modulation of the intrarenal renin-angiotensin system (Doctoral dissertation, UTAR).
- Balart-García, P., Aristide, L., Bradford, T., Beasley-Hall, P., Polak, S., Ribera, I., … & Fernandez, R. (2022). Genomic exaptation and convergent evolution paved the way to independent subterranean colonization across beetle lineages.
- Luparello, C., Branni, R., Abruscato, G., Lazzara, V., Drahos, L., Arizza, V., … & Vazzana, M. (2022). Cytotoxic capability and the associated proteomic profile of cell-free coelomic fluid extracts from the edible sea cucumber Holothuria tubulosa on HepG2 liver cancer cells. EXCLI Journal, 21, 722-743.
- López-Grueso, M. J., Lagal, D. J., García-Jiménez, Á. F., Tarradas, R. M., Carmona-Hidalgo, B., Peinado, J., … & Padilla, C. A. (2020). Knockout of PRDX6 induces mitochondrial dysfunction and cell cycle arrest at G2/M in HepG2 hepatocarcinoma cells. Redox Biology, 101737.
- Criel, H. (2019). INTRACELLULAR ACCUMULATION OF QUERCETIN AND ITS IMPACT ON CELLULAR STRESS: AN IN VITRO STUDY (Doctoral dissertation, Ghent University).
- Luparello, C., Asaro, D. M. L., Cruciata, I., Hassell-Hart, S., Sansook, S., Spencer, J., & Caradonna, F. (2019). Cytotoxic Activity of the Histone Deacetylase 3-Selective Inhibitor Pojamide on MDA-MB-231 Triple-Negative Breast Cancer Cells. International Journal of Molecular Sciences, 20(4), 804.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q: What is the probe used in this kit?
A: H2DCFDA, a cell-permeant fluorescent probe.
Q: What is the detection principle?
A: Oxidation by ROS produces highly fluorescent DCF.
Q: Is the kit radioactive?
A: No, it is non-radioactive and safe to use.
5 reviews for ROS Detection Assay Kit
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Pieter Baert –
I am very pleased with the ROS Detection Assay Kit, as the entire buying experience was simple and professional. Shipping was prompt, and the product quality is excellent, meeting all the specifications listed on the website. This kit helped me overcome challenges with detecting low levels of oxidative activity in treated cell lines. It has significantly improved the efficiency of my workflow and ensured that my experimental conclusions are based on solid data. This is a dependable tool for anyone researching cellular health and stress responses.
Sandrine Leroy –
Ordering this assay kit was a great experience as everything was clearly described and the checkout was very user-friendly. The kit arrived quickly and was well-packaged to maintain the stability of the fluorescent probes. It effectively solved the issues I was having with high background noise in my flow cytometry experiments, allowing for much clearer results. The protocol was easy to follow, and I was able to get my results much faster than with other methods I’ve tried. I highly recommend this product for its precision and reliability in cell-based assays.
Simon Gijsbrechts –
My purchase of the ROS Detection Assay Kit was a positive experience, with a straightforward ordering process and very fast shipping to Brussels. The kit is well-designed, and the product description accurately reflected its performance in the lab. It helped me solve my issue with quantifying oxidative stress in primary cell cultures, providing a clear and measurable output. The results were consistent and significantly contributed to the success of my current project. I am giving it four stars instead of five only because I would appreciate a smaller trial size for quick pilot tests, but otherwise, the kit is excellent.
Isabelle Claes –
Finding a reliable ROS detection kit was easy thanks to the detailed information and clear instructions provided on this site. The delivery was fast, and the kit worked perfectly right out of the box for my microplate reader assays. It solved my problem of reagent degradation, as the H2DCFDA probe provided very stable and consistent readings throughout my experiments. I appreciate how easy the whole process was, from the initial purchase to the final analysis of my samples. I will certainly be ordering this kit again for my future research projects.
Dirk Van Damme –
I purchased the ROS Detection Assay Kit for my study on oxidative stress in cancer cells, and the ordering process was extremely smooth. The website provided very clear technical descriptions, and the delivery to my lab in Brussels was remarkably fast and secure. This kit genuinely helped me by providing a sensitive and reliable way to measure intracellular ROS levels using the DCFDA method. It solved my previous problem with inconsistent fluorescence signals, giving me much more accurate and reproducible data. I am very satisfied with this purchase and the high quality of the reagents.