SOD Activity Assay Kit

Easy and fast quantitative determination of SOD enzyme activity.

The SOD Activity Assay Kit provides a fast and reliable method for the quantitative determination of Superoxide Dismutase (SOD) enzyme activity.

In this assay, superoxide ions are generated from the xanthine–xanthine oxidase system. These superoxide ions convert the tetrazolium salt WST-1 into a colored formazan product, measured at 450 nm. Addition of SOD reduces superoxide levels, decreasing formazan formation. SOD activity is calculated as the percent inhibition of WST-1 formazan generation.

With a streamlined protocol, ready-to-use reagents, and high reproducibility, this kit is ideal for oxidative stress research, antioxidant screening, and enzymatic activity assays.

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Price: 286.90 

SKU: CA061 Categories: ,

Detailed information:

Advantages & Features

  • Sensitive and accurate quantitative assay.

  • Simple workflow, compatible with microplate readers.

  • Fast protocol, <40 minutes.

  • Provides 100 assays per kit.

  • Strict lot-to-lot consistency.

Specifications

Specification Description
Detection Colorimetric (450 nm, microplate reader)
Principle Inhibition of WST-1 formazan formation by SOD
Protocol time <40 minutes
Includes WST-1 reagent, buffers, SOD enzyme, SOD standard
Storage 4 ºC (buffers), –20 ºC (SOD standard)

Includes

– 1 mL WST-1 Reagent
– 20 mL SOD assay buffer
– 10 mL SOD dilution buffer
– 30 µL SOD enzyme solution
– 1 mg SOD standard (3000 U/mg)

Download documentation

Datasheet
MSDS

Applications

  • Measurement of SOD activity in biological samples.

  • Oxidative stress studies.

  • Antioxidant screening.

  • Drug discovery and biochemical assays.

Tables & Figures

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Quality Control

  • Manufactured under strict QC to guarantee sensitivity and reproducibility.

  • Validated with SOD enzyme standard controls.

Advice

  • Protect WST-1 reagent from light (store in amber tubes).

  • Always include positive control for assay validation.

Storage, Shipping & Guarantee

  • Shipping: Gel pack.
  • Storage: The SOD Standard should be stored at ‐20 °C. All other components of this kit are stable at 4 °C until the kit expiration date.
  • Recommendations: WST-1 reagent is light sensitive and should be maintained in amber tubes.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

FAQs

Q: How is SOD activity measured?
A: As the percent inhibition of WST-1 formazan formation at 450 nm.

Q: How many reactions are included?
A: 100 assays per kit.

Q: What is the storage condition?
A: Store buffers at 4 ºC, SOD standard at –20 ºC.

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