6X OX/Loading Buffer
Ready-to-use Orange G and Xylene Cyanol loading dye for accurate DNA gel electrophoresis tracking.
6× OX Loading Buffer from Canvax™ is a ready-to-use DNA loading dye containing Orange G and Xylene Cyanol FF, specifically formulated for accurate visualization of DNA migration during agarose gel electrophoresis. This dual-dye formulation provides clear and distinct tracking fronts across a wide range of DNA fragment sizes, simplifying electrophoresis monitoring and improving experimental precision.
The buffer’s optimized density ensures samples load evenly into wells, reducing pipetting errors and guaranteeing consistent band sharpness and reproducibility.
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Price: 31.00 €
Detailed information:
Advantages & Features
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Dual tracking dyes: Combines Orange G and Xylene Cyanol FF for precise migration monitoring.
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Ready-to-use formulation: No dilution required—mix directly with DNA samples.
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High density: Enables easy loading and minimizes sample dispersion in gel wells.
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Compatible with TAE and TBE buffers: Suitable for standard agarose gel electrophoresis systems.
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Non-interfering: Dyes do not affect DNA recovery, ligation, or downstream enzymatic reactions.
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Stable formulation: Long-term stability under ambient storage conditions.
Specifications
- Concentration: 0.15% orange G, 0.03% xylene cyanol FF, 60% Glycerol, 10 mM Tris-HCl (pH 7.6) and 60 mM EDTA.
Includes
-5 x 1 mL of selected Loading Buffer
Download documentation
Datasheet MSDSApplications
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DNA gel electrophoresis.
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Monitoring DNA migration in agarose gels.
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PCR product and restriction digest analysis.
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Optimization of DNA separation.
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Routine molecular biology workflows.
Tables & Figures
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Quality Control
- Each batch is tested for consistent dye migration, absence of nuclease contamination, and optical clarity.
Advice
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Mix 1 volume of 6× OX Loading Buffer with 5 volumes of DNA sample.
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Avoid overfilling gel wells to ensure sharp bands.
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Run gels alongside molecular weight markers for accurate fragment tracking.
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Store tightly closed to prevent evaporation.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: 4 °C.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What are the main tracking dyes in 6× OX Loading Buffer?
It contains Orange G and Xylene Cyanol FF, migrating approximately at 50 bp and 4 kb respectively in 1 % agarose gels.
Q2: Can it be used for RNA electrophoresis?
Yes, the formulation is suitable for both DNA and RNA electrophoresis in non-denaturing conditions.
Q3: Does the buffer affect downstream DNA purification?
No, the dyes do not interfere with gel extraction or enzymatic manipulation.
