T4 DNA Polymerase (5 U/µL)
High-fidelity enzyme for DNA blunting, filling-in, and labeling reactions in molecular cloning.
T4 DNA Polymerase is an enzyme with 5′→3′ polymerase and 3′→5′ exonuclease activities, commonly used for DNA end repair and blunt-end formation. It delivers precise enzymatic activity and reliable performance in cloning, sequencing, and labeling reactions.
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Price: 312.12 €
Detailed information:
Advantages & Features
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High fidelity: Strong 3’→5’ exonuclease proofreading activity ensures accurate end processing.
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Dual functionality: Performs both polymerization and exonuclease reactions for efficient DNA end repair.
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Ready-to-use format: Supplied at 5 U/µL in optimized storage buffer for direct application.
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Reliable blunting efficiency: Produces clean, blunt-ended DNA fragments for cloning and labeling.
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Compatible with multiple workflows: Ideal for blunt-end cloning, probe preparation, and second-strand cDNA synthesis.
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Stable and reproducible: Manufactured under controlled conditions to guarantee batch-to-batch consistency.
Specifications
- Unit definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37 °C using DNAse I-Nicked DNA as template-primer.
- Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 1 mM DTT, 50 % glycerol
Includes
– T4 DNA Polymerase (5 U/μL)
– Reaction Buffer (5x)
Download documentation
Datasheet MSDSApplications
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Blunt-end DNA preparation for cloning and ligation.
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Removal of 3′ overhangs in restriction fragments.
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Fill-in of recessed 3′ ends.
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Labeling of DNA fragments using radioactive or fluorescent nucleotides.
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Second-strand cDNA synthesis during library construction.
Tables & Figures
Quality Control
- Each lot is tested for nuclease contamination, specific activity, and functional performance in DNA blunting and labeling assays.
Advice
- Use freshly thawed enzyme aliquots to maintain activity.
- Avoid repeated freeze–thaw cycles.
- For optimal blunting, incubate at 12–16 °C for 15–30 min and terminate reactions by heat inactivation or EDTA addition.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: Can T4 DNA Polymerase be used to remove 3′ overhangs?
A: Yes. Its strong 3′→5′ exonuclease activity efficiently removes single-stranded 3′ extensions.
Q2: Is the enzyme compatible with radioactive labeling?
A: Absolutely. It supports incorporation of labeled nucleotides at recessed 3′ ends.
Q3: What buffer conditions are recommended?
A: Use the supplied reaction buffer or any standard T4 polymerase buffer containing Mg²⁺ and dNTPs.
