Bovine Factor Xa

Serine protease for specific cleavage of fusion tags in recombinant proteins.

Bovine Factor Xa is a high-purity serine protease used for cleaving fusion proteins that contain a Factor Xa recognition site. It provides specific and reproducible cleavage, minimizing unwanted degradation.

The enzyme ensures efficient processing under mild conditions. It is supplied in a ready-to-use format and delivers consistent performance for protein purification and biochemical assays.

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Price: 342.02 

SKU: P0260 Categories: ,

Detailed information:

Advantages & Features
  • High specificity: preferential cleavage at Ile-(Glu or Asp)-Gly-Arg sites.

  • Efficient tag removal: widely used for recombinant protein purification.

  • Proven reliability: purified from bovine plasma and fully activated.

  • Consistent performance: manufactured under strict QC standards.

  • High purity: ≥95% confirmed by SDS-PAGE.

Specifications
Parameter Specification
Source Bovine plasma
Specific Activity >210 U/mg
Extinction Coefficient 12.4 (1%)
Molecular Weight 45.3 kDa
Purity ≥95% SDS-PAGE
Storage Buffer 20 mM Tris-HCl, 0.7 M NaCl, pH 7.4
Unit Definition One unit of activated Factor X will liberate 1.0 μmole of p-nitroanilide from a synthetic Ile-Glu-Gly-Arg peptide with p-nitroanilide derivatized arginine as the substrate per minute at pH 8.3 at 37 °C.
Volume 250 µg (1 mg/mL)
Includes

250 µg Bovine Factor Xa (1 mg/mL).

Download documentation
Datasheet
MSDS
Applications
  • Fusion tag removal from recombinant proteins.

  • Protein purification and processing.

  • Precise cleavage of engineered recognition sites.

  • Biochemical and structural protein studies.

Tables & Figures

Quality Control
  • Specific Activity: >210 U/mg.

  • Purity ≥95% by SDS-PAGE.

  • Manufactured under strict QC for lot-to-lot consistency.

Advice
  • Activated using RVV-X (removed after activation).

  • Do not use if cleavage site contains proline or arginine after Arg.

  • Ensure storage at ultra-low temperature for long-term stability.

Storage, Shipping & Guarantee
  • Shipment Dry Ice.
  • Storage: -70°C.
Citations
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review
FAQs

Q1: Can Bovine Factor Xa cleave all proteins?
No, it cleaves specifically after arginine in Ile-(Glu or Asp)-Gly-Arg sequences.

Q2: Is the enzyme completely activated?
Yes, complete activation is confirmed by SDS-PAGE.

Q3: Can Bovine Factor Xa be reused?
No, but it can be removed after cleavage by affinity chromatography.

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