ONPG – Galactosidase Assay Kit

Optimized, Stable & Convenient tool to quickly measure the levels of active β-galactosidase

ONPG B-Galactosidase Assay Kit is an optimized, stable and convenient tool to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing Lac Z.

Lac Z is often used reporter gene in experiments transfection because the β-galactosidase is very resistant to proteolytic degradation and its activity is easily measured. β-galactosidase performs the hydrolysis of orthonitrophenyl-β-D-galactopyranoside (ONPG) to the ortho-nitrophenol (ONP). This ONP produces a bright yellow colour that is detected at an absorbance of 420 nm. The concentration of β-galactosidase is proportional to colour produced.

Price: 228.01 

Detailed information:

Advantages & Features
  • Proven performance to quantify high expression level of beta-Gal.
  • Very stable: resistant to proteolytic degradation and easily assayed.
  • Convenient for all transfection assays.
  • Versatile: proven performance for cultured cells and tissues.
  • Fast and easy protocol.
  • Cost-effective.

– 10 mL ONGP Substrate solution (4mg/ml)
– 0.5 ml β-mercaptoethanol
– 10 mL Buffer Lysis
– 10 mL Buffer Assay (10x)
– 70 mL Buffer Stop Solution
– 100 μl β-galactosidase enzyme (0.4U/μl)

Download documentation
  • Measurement of β-Galactosidase activity in the lysates of transfected cells.
Tables & Figures

Quality Control
  • Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
Storage, Shipping & Guarantee
  • Shipped in: Gel Pack.
  • Storage: 4 ºC.
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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