PRImeDETECT™ STEC Detection Kit

For a Fast & Reliable Amplification and Detection of specific DNA fragments from Sof the virulence-associated genes stx1, stx2 and eae by the real-time PCR

PRImeDETECT™ STEC Detection Kit is an ideal tool for an specific, rapid, and reliable Amplification and Detection of the virulence-associated genes stx1, stx2 and eae by the real-time PCR method (Multiplex PCR).

The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, MgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors.

Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The Probe for the detection of target gene is labelled with the FAM (sxt1), HEX (stx2) and ROX (eae) fluorochrome, whereas the probe for the detection of IAC is labelled with the CY5 fluorochrome.

In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.

Price: 640.76 

SKU: FP0036 Category:

Detailed information:

Advantages & Features
  • Easy and Ready-to-use Kit.
  • Fast: results in less than 24 hours with minimal handling.
  • Reliable and highly specific Results.
  • High Quality: manufactured according strict quality controls in every manufacturing step to guarantee the highest reproducibility.
  • Customizable Multiplex Solutions: according to your requirements, to measure up to 5 Pathogens of your choice at the same time in 1 kit. Ask us how now.
  • Amplification limit:  5 DNA copies per reaction.
  • Quantification Dynamic range: 4 logs.
  • Simultaneously detect and differentiate the major STEC-associated virulence marker genes: stx1, stx2 and eae in the same PCR amplification reaction.
  • Inclusivity: specifically reacts with all strains of E. coli with the indicated target genes (stx1, stx2 and eae).
  • Compliance: Manufactured according to ISO/TS 13136:2012.
  • Exclusivity: No cross-reactions with other strains of Non- Shiga toxin-producing E. coli (STEC).
  • Detection: probe labelled with fluorescent dyes–  FAM-BHQ1a (sxt1), HEX-BHQ1(stx2), ROX-BHQ2 (eae) and CY5-BHQ1(IAC).
  • Thermal cycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers.

– 1 x Multiplex PCR MasterMix
– 1 x DNAready Lysis Buffer
– 1 x PCR Positive Control
– 1 x PCR Negative Control

Download documentation
Tables & Figures

Quality Control
  • Manufactured according to ISO/TS 13136:2012.
  • Strict quality controls in every manufacturing step to guarantee the highest quality and reproducibility.
Storage, Shipping & Guarantee
  • Shipment at Ambient Temperature.
  • Storage: -20 °C upon receipt. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month.
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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