DNA Suspension Buffer (pH 8.0)

Sterile, nuclease-free buffer at pH 8.0, ideal for DNA resuspension, storage, PCR, sequencing, and cloning.

The DNA Suspension Buffer (pH 8.0, sterile) is a high-purity, nuclease-free solution designed for optimal DNA stability and integrity. It provides ideal conditions for DNA resuspension, storage, and downstream applications such as PCR, qPCR, sequencing, cloning, and transformation.

Formulated with Tris and EDTA, it prevents DNA degradation and ensures reproducibility in sensitive molecular biology workflows. Available in 1 L and 5 L sterile formats, it is manufactured under ISO 9001 in an ISO 13485 & GMP-certified facility, ensuring batch-to-batch consistency.

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Price: 45.00 180.00 

SKU: BR0375 Categories: ,

Detailed information:

Advantages & Features
  • Sterile
  • Reliable: Manufactured under rigorous quality control standards to ensure consistent performance across batches.
  • High Purity: Free from DNase, RNase, and protease activities, preserving sample integrity.
  • Time-Saving: Ready-to-use format minimizes experiment preparation time, enhancing efficiency.
  • Comprehensive and Versatile Solution
Specifications
  • Grade: PCR
  • Format: Liquid
  • DNAse/RNAse Free tested.
  • Composition (1M): WFI Water (99,78%);  Tris (0,1211%); Hydrochloric Acid, 6N (0,1%) and EDTA Disodium Dihydrate (0,0037%)
  • Sterilization: purified using a 0.22 µm filtration process [aseptically filled]
Includes
  • Includes for 1L: 1,000 mL solution in PET Bottle
  • Includes for 5L: 5L solution in 5L graduated square containers with moulded hand grip.
Download documentation
Datasheet
MSDS
Applications

Designed for the most commonly used Research and Biomanufacturing applications, such as:

  • DNA resuspension and storage.

  • PCR and qPCR preparation.

  • Next-Generation Sequencing (NGS).

  • Cloning and transformation.

Tables & Figures

Quality Control

Manufactured in an ISO 13485:2016 and GMP-certified facility, located in Boecillo, Valladolid (Spain), under ISO9001:2016 procedures to ensure the highest quality standards. Our processes include:

    • Batch-to-batch consistency.
    • Facilities audited regularly Quality audits.
    • Lot specific CoAs and QA review previously to release a product.
    • Material traceability, documented procedures and documented employee training.
    • Equipment maintenance and monitoring records.

Request a Certificate of Analysis (CoA)

Advice
  • Use sterile techniques when handling.

  • Avoid repeated freeze-thaw cycles.

Storage, Shipping & Guarantee
  • Shipping: Ambient Temperature.
  • Storage: Room Temperature or at 4 ºC.
Citations
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review
FAQs

Q: What is the composition of the DNA Suspension Buffer?
A: Tris (10 mM), EDTA (0.1 mM), pH 8.0 in sterile WFI water.

Q: Is it nuclease-free?
A: Yes, free from DNase, RNase, and protease.

Q: Can it be used for PCR or sequencing?
A: Yes, validated for PCR, qPCR, sequencing, cloning, and NGS.

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