TAE (pH 8.3)
Ready-to-use sterile TAE Buffer (pH 8.3) for DNA electrophoresis and recovery.
The TAE Buffer (Tris-Acetate-EDTA, pH 8.3) is a high-quality, ready-to-use solution designed for nucleic acid electrophoresis. With low ionic strength, it enables efficient separation of DNA fragments larger than 1,500 bp while preserving DNA integrity for downstream recovery.
It is widely used in agarose and polyacrylamide gel electrophoresis, PCR product analysis, DNA purification, and cloning. Supplied sterile and free of DNases, RNases, and proteases, TAE Buffer ensures reproducible, contamination-free performance.
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Price: 29.80 € – 88.30 €
Detailed information:
Advantages & Features
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Reliable: free of DNase, RNase, and protease activity.
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Efficient DNA migration with low ionic strength.
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High-quality electrophoresis results for fragments >1,500 bp.
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Convenient: supplied in 10x and 50x formats.
- Sterilization: purified using a 0.22 µm filtration process [aseptically filled].
Specifications
- Chemicals: Analytical grade.
- Format: Ready-to-use solution
- RNAse/DNAse activity: non-detectable.
- Composition (10x): 40 mM Tris, 20 mM Acetic Acid and 1mM EDTA (1x)
- pH: 8.3± 0.05 at 25 ºC.
- Sterilization: purified using a 0.22 µm filtration process [aseptically filled].
Includes
1,000 mL TAE (10x or 50x) Ready-to-use solution
Download documentation
Datasheet MSDSApplications
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DNA electrophoresis in agarose and polyacrylamide gels.
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PCR product analysis.
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DNA cloning and purification workflows.
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Transfer buffer in Northern blotting.
Tables & Figures
Quality Control
- Manufactured in an ISO 13485:2016 and GMP-certified facility, located in Boecillo, Valladolid (Spain), under ISO9001:2016 procedures to ensure the highest quality standards. Our processes include:
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- Batch-to-batch consistency.
- Facilities audited regularly Quality audits.
- Lot specific CoAs and QA review previously to release a product.
- Material traceability, documented procedures and documented employee training.
- Equipment maintenance and monitoring records.
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Advice
- Recommendations: Store the product in a dry place.
Storage & Shipping
- Shipped in:Â Ambient Temperature.
- Storage:Â Room Temperature.
Citations
- Gómez-Melero, S. (2022). Desarrollo de anticuerpos terapéuticos contra el receptor CCR6 humano.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
TAE buffer solution, Tris acetate EDTA buffer, DNA electrophoresis buffer TAE, Agarose gel buffer TAE, TAE running buffer
Also known as:
- Spanish: Buffer TAE pH 8.3, Tampón Tris-acetato-EDTA, Buffer de electroforesis ADN
- French: Tampon TAE pH 8.3, Tampon Tris-acétate-EDTA, Tampon électrophorèse ADN
- German: TAE-Puffer pH 8.3, Tris-Acetat-EDTA-Puffer, DNA-Elektrophoresepuffer
- Italian: Tampone TAE pH 8.3, Tampone Tris-acetato-EDTA, Tampone elettroforesi DNA
FAQs
Q: What formats are available?
A: 10x and 50x.
Q: What is the recommended application?
A: DNA electrophoresis, PCR product analysis, and cloning.
1 review for TAE (pH 8.3)
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Benjamin Clarke –
We use this buffer for routine DNA electrophoresis during cloning projects. The concentrate is practical to store and preparing working solutions is quick, making daily laboratory work more efficient.