TBE (pH 8.3) (10x)

Sterile, ready-to-dilute 10× TBE buffer (pH 8.3) for reliable DNA and RNA electrophoresis.

TBE Buffer (pH 8.3, 10×) from Canvax™ is a sterile, ready-to-dilute electrophoresis buffer optimized for DNA and RNA separation in agarose and polyacrylamide gels. Its formulation of Tris base, boric acid, and EDTA ensures high ionic strength, efficient migration, and superior resolution of nucleic acid fragments during electrophoretic analysis.

Produced in Canvax’s ISO 13485 and GMP-certified facility under ISO 9001 standards, this high-purity buffer provides excellent sterility, reproducibility, and long-term stability, ensuring consistent results in sensitive molecular biology workflows such as cloning, PCR product analysis, and sequencing.

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Product available under ISO13485 / cGMP or with custom formulation, packaging or application. Learn more >

Price: 55.83 

SKU: BR0030 Categories: ,

Detailed information:

Advantages & Features
  • Sterile and ready-to-dilute: Eliminates the need for filtration or preparation.

  • High-quality manufacturing: Produced in an ISO 13485 and GMP certified plant, under an ISO 9001 standard for maximum reliability.

  • Reliable electrophoresis performance: Ensures sharp, well-resolved DNA and RNA bands.

  • High ionic strength: Provides efficient current flow and reproducible migration.

  • Excellent purity: Free from DNase, RNase, and protease contamination.

Specifications
  • Chemicals: Analytical grade.
  • Format: 10x solution
  • Concentration: 0.89M Tris-Borate, 0.02M EDTA (10x)
  • Sterilization: purified using a 0.22 µm filtration process [aseptically filled].
  • pH: 8.3 ± 0.15 at 25°C
  • Appearance: Clear, colorless solution
  • Purity: DNase-, RNase-, and protease-free
Includes

– 1,000 ml TBE (10x) (pH 8.3) ready-to-use solution

Download documentation
Datasheet
MSDS
Applications
  • Agarose gel electrophoresis of DNA and RNA.

  • Polyacrylamide gel electrophoresis (PAGE).

  • PCR product analysis.

  • Restriction digestion verification.

  • Molecular cloning and sequencing workflows.

Tables & Figures

Quality Control
  • Each batch is tested for pH accuracy, conductivity, sterility, and absence of DNase, RNase, and protease activity to ensure reproducible electrophoretic performance.

  • Manufactured in an ISO 13485:2016 and GMP-certified facility, located in Boecillo, Valladolid (Spain), under ISO9001:2016 procedures to ensure the highest quality standards. Our processes include:
      • Batch-to-batch consistency.
      • Facilities audited regularly Quality audits.
      • Lot specific CoAs and QA review previously to release a product.
      • Material traceability, documented procedures and documented employee training.
      • Equipment maintenance and monitoring records.

    Request a Certificate of Analysis (CoA)

Advice
  • Dilute 1 part of 10× buffer with 9 parts of deionized water to prepare 1× working solution.

  • Use freshly diluted buffer for optimal resolution.

  • Avoid reusing running buffer to maintain performance consistency.

Storage, Shipping & Guarantee
  • Shipped at: Ambient Temperature.
  • Storage: Room temperature.
Citations
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review
FAQs

Q1: What is the recommended dilution ratio?
Dilute 1:9 with deionized water to obtain a 1× working solution for electrophoresis.

Q2: Is the buffer sterile?
Yes. It is 0.2 µm filtered to ensure sterility and reliability in sensitive applications.

Q3: Can it be used for both DNA and RNA gels?
Yes. TBE Buffer supports high-resolution electrophoresis of both nucleic acids.

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