SEAP Reporter Gene Assay Kit

– 3 x 96 W Solid Plate (white)
– 3 units of lid
– 50 μL Alkaline Phosphatase Standard
– 15 mL SEAP Substrate (Luminescence)

• Reporter gene assays.

• Promoter and enhancer activity studies.

• Signal transduction pathway analysis.

• Transfection efficiency evaluation.

• Each lot is validated for sensitivity, reproducibility, and signal-to-noise ratio.

• Use clear, cell-free supernatants for accurate readings.

• Handle reagents at room temperature to optimize enzyme activity.

• Avoid contamination between wells when using multiwell plates.

• Shipping: Ambient Temperature.
• Storage:  4 ºC, except Component solution containing CSPD® Substrate (2-6 °C), Placental Alkaline Phosphatase Positive Control (-20 °C).
• Recommendations: Protect SEAP Substrate from light.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q1: What does SEAP stand for?
SEAP stands for Secreted Alkaline Phosphatase, a common reporter enzyme used in gene expression studies.

Q2: Is this assay destructive to cells?
No, it allows non-destructive measurement of SEAP in the culture medium.

Q3: Which instruments are compatible?
It can be read on standard luminometers or microplate readers using luminescent or colorimetric detection.