DNA Polymerase I (E. coli) (10 U/µL)
Recombinant E. coli enzyme for DNA repair, labeling, and second-strand synthesis.
DNA Polymerase (E. coli) is a DNA-dependent DNA polymerase possessing 5′→3′ polymerase, 3′→5′ proofreading exonuclease and 5′→3′ exonuclease activities. It is widely used for nick translation, second–strand cDNA synthesis, DNA repair and gap filling in molecular biology applications.
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Price: 94.90 €
Detailed information:
Advantages & Features
- Dual enzymatic activity: Performs both polymerase and exonuclease reactions for efficient DNA repair and synthesis.
- High purity: Free of endonuclease and RNase contamination for reproducible results.
- Optimized for nick translation: Ensures uniform labeling of DNA fragments with fluorescent or radioactive nucleotides.
- Ready-to-use concentration: Supplied at 10 U/µL for direct application without dilution.
- Compatible with diverse workflows: Ideal for cDNA synthesis, DNA labeling, and strand displacement assays.
Specifications
- Storage Buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 1 mM DTT, 50 % glycerol
- Activity definition: 1 U incorporates 10 nmol of dNTPs into acid-insoluble form in 30 min at 37 °C
Includes
– 50 μL DNA Polymerase I (10 U/μL)
– 1 mL Reaction Buffer (10x)
Download documentation
Datasheet MSDSApplications
- Nick translation for labeled DNA probe synthesis.
- Second-strand cDNA synthesis.
- DNA repair and gap filling.
- Removal of RNA primers during DNA replication.
- Molecular cloning and sequencing workflows.
Tables & Figures
Quality Control
- Each batch is tested for specific activity, purity, and absence of contaminating nucleases. Functional assays confirm polymerase and exonuclease performance in nick translation reactions.
Advice
- Thaw on ice and mix gently before use.
- Avoid multiple freeze–thaw cycles.
- For labeling reactions, use recommended dNTP concentrations and include Mg²⁺ cofactor.
- Heat-inactivate at 70 °C for 10 min to stop the reaction.
Storage & Shipping
- Shipped in: Gel pack.
- Storage: -20 °C.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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Synonym(s)
DNA polymerase I enzyme, E. coli DNA polymerase I, DNA synthesis enzyme, Nick translation enzyme, DNA repair polymerase
Also known as:
- Spanish: ADN polimerasa I de E. coli, Enzima de síntesis de ADN, Enzima de reparación de ADN
- French: ADN polymérase I E. coli, Enzyme de synthèse ADN, Enzyme de réparation ADN
- German: DNA-Polymerase I aus E. coli, DNA-Syntheseenzym, DNA-Reparaturenzym
- Italian: DNA polimerasi I di E. coli, Enzima sintesi DNA, Enzima riparazione DNA
FAQs
Q1: What are the main applications of DNA Polymerase I (E. coli)?
It is primarily used for nick translation, DNA labeling, and second-strand cDNA synthesis.
Q2: Does the enzyme exhibit proofreading activity?
Yes. It has intrinsic 3′→5′ exonuclease activity that enhances fidelity during synthesis.
Q3: Can DNA Polymerase I be replaced by the Klenow fragment?
The Klenow Fragment is suitable for applications that require polymerase and proofreading activities only. Full-length DNA Polymerase I retains the additional 5′→3′ exonuclease activity required for applications such as nick translation.
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