DNase I Set, RNase-free

• Unit Definition: One Kunitz unit is defined as the amount of DNase I that causes an increase in A₂₆₀ of 0.001 per minute per ml at 25 °C, pH 5.0, with highly polymerized DNA as substrate.

Includes for 50 preps:
– 250 μL DNase I (2U*/ μL)
– 2.5 mL OM Buffer

*Unit Definition ((Kunitz): One Kunitz unit is defined as the amount of DNase I that causes an increase in A260 of 0.001 per minute per ml at 25 °C, pH 5.0, with highly polymerized DNA as substrate.

• Removal of DNA contamination in RNA samples.

• Preparation of RNA for RT-PCR and qPCR.

• RNA sequencing workflows.

• Northern blot and transcript analysis.

• Each lot is tested to confirm RNase-free quality, high enzymatic activity, and absence of proteases.

• Use freshly prepared reaction mixes for maximum efficiency.

• Inactivate DNase I after treatment to protect RNA for downstream use.

• Store enzyme in aliquots to avoid repeated freeze-thaw cycles.

• Shipped in: Gel pack.
• Storage: DNase I at -20°C and OM Buffer at Room Temperature.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q1: Is this enzyme guaranteed RNase-free?
Yes, it is rigorously tested to be RNase-free and safe for RNA workflows.

Q2: What is the main application of this kit?
It is used to remove contaminating DNA from RNA samples prior to RT-PCR, qPCR, or sequencing.

Q3: How is the enzyme supplied?
It is provided with an optimized reaction buffer for reliable performance.