RNase A, >5,000 U worthinton/mg, 10mg/mL

High-purity RNase A solution (5,000 U/mg, 10 mg/mL) for RNA removal in DNA prep.

RNase A Solution is a highly purified endoribonuclease that specifically cleaves single-stranded RNA at the 3′ side of pyrimidine residues. It is widely used in molecular biology workflows for the removal of RNA during DNA preparation and for the analysis of RNA structure.

This enzyme is free of DNase and protease contamination, ensuring reliable performance in plasmid preparation, genomic DNA extraction, and hybridization experiments. The RNase A solution is ready-to-use, stable, and suitable for demanding laboratory applications.

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Price: 39.60 

SKU: EZ0002 Categories: ,

Detailed information:

Advantages & Features

  • High purity: free of DNase and protease contamination.

  • High activity: 5,000 Worthington units/mg ensures efficient RNA degradation.

  • Reliable performance: validated for molecular biology workflows.

  • Ready-to-use solution: supplied at 10 mg/mL for convenience.

  • Versatile applications: DNA purification, RNA analysis, hybridization assays.

Specifications

Unit definition:
One unit of the enzyme causes an increase in absorbance of 1.0 at 260 nm when yeast RNA is hydrolyzed at 37 °C and pH 5.0. 50 units are approximately equivalent to 1 Kunitz unit.

Note: One Kunitz unit equals to 50 Worthington units.

Includes

– 1 mL RNase A Solution (10 mg/mL)

Download documentation

Datasheet
MSDS

Applications

  • Removal of RNA during DNA purification.

  • Plasmid and genomic DNA preparation.

  • RNA structural analysis.

  • Hybridization assays.

Tables & Figures

Quality Control

  • Each lot is tested for activity, concentration, and absence of DNase and protease contamination.

Advice

  • Avoid introducing RNase A into RNA workflows to prevent unwanted degradation.

  • Use recommended aliquots to minimize freeze-thaw cycles.

  • Inactivate enzyme when required by heating or chelation.

Storage, Shipping & Guarantee

  • Shipped in: Gel pack.
  • Storage: RNase A solutions retain enzymatic activity at room temperature or 2-8 ºC for extended periods. However store at –20 ºC in a freezer without a defrost cycle is recommended. Avoid freeze/thaw cycles.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

FAQs

Q1: What is the main application of this RNase A?
It is used to remove RNA contamination during DNA extraction and preparation.

Q2: Is it free of DNase contamination?
Yes, it is rigorously tested to be DNase- and protease-free.

Q3: How is the enzyme supplied?
It is provided as a ready-to-use 10 mg/mL solution.

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