Proteinase K Solution
Ready-to-use Proteinase K solution 20 mg/mL, nuclease-free, for protein digestion in DNA/RNA extraction and molecular biology workflows.
The Proteinase K Solution 20 mg/mL is a highly active, broad-spectrum serine protease purified from Tritirachium album. This ready-to-use Proteinase K solution cleaves peptide bonds adjacent to aliphatic and aromatic amino acids, making it ideal for digesting proteins during DNA and RNA extraction workflows.
Its stability at elevated temperatures and resistance to detergents such as SDS and chelating agents like EDTA ensure reliable performance under denaturing conditions. Supplied in 50% glycerol buffer for convenient handling, the Proteinase K solution guarantees reproducibility, nuclease-free purity, and consistent results in molecular biology protocols.
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Price: 44.40 €
Detailed information:
Advantages & Features
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High activity: 20 mg/mL, ≥30 units/mg.
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Stable at high temperatures and under denaturing conditions.
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Nuclease-free: DNase, RNase, and exonuclease not detected.
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Convenient: liquid format in 50% glycerol buffer.
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Reliable: batch-to-batch consistency ensured.
Specifications
Storage Buffer: 50 mM Tris, pH 8, 3 mM CaCl2, glycerol 50%
Unit Definition: Unit definition One unit is the amount of enzyme which releases at 37 °C in 1 min as many folin-positive amino acids and peptides from hemoglobin as 1 μmol of tyrosine.
Includes
– 1 mL Proteinase K Solution (20 mg/mL); Ready-to-use. Storage Buffer: 50 mM Tris, pH 8, 3 mM CaCl2, glycerol 50%
Download documentation
Datasheet MSDSApplications
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Genomic DNA extraction from cells and tissues.
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RNA isolation free of nuclease contamination.
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Protein digestion in molecular biology protocols.
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Enzyme localization studies.
Tables & Figures
Quality Control
- Enzyme activity is assayed by digesting hemoglobin at a concentration of 16.7 mg/mL in a solution of 0.08 M potassium phosphate (pH 7.5), 5 M urea, 4 mM NaCl, 3 mM CaCl2.
- Free of detectable RNase, DNase and exonuclease activities.
Advice
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Mix gently before use, avoid frothing.
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Do not heat-inactivate unless required by protocol.
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Use RNase-free consumables for nucleic acid workflows.
Storage, Shipping & Guarantee
- Shipment at Ambient Temperature.
- Storage: 4 °C or -20 °C.
Citations
- Córdoba, A. D. J. R., Zambrano, J. C., RodrÃguez, Y. G. M., Oliva, K. J. O., Cano, A. V. M., Vasconez, P. A. V., & Ramos, J. A. R. (2025). Influence of A1/A2 allelic variants of the CSN2 gene on milk composition and production in Holstein cows from Nariño, Colombia. Revista de la Facultad de Medicina Veterinaria y de Zootecnia, 72(2), 1-15.
- Riascos-España, A. F., Cuastumal, B. T. A., Zambrano, M. C. I., Arteaga, J. Z. C., & Velasquez-Vasconez, P. A. (2025). Optimized Protocol for DNA Extraction in Three Theobroma Species. Bio-protocol, 15(9), e5297.
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Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
Proteinase K enzyme solution, Protein digestion enzyme, Protease K solution, Broad-spectrum protease, Protein degradation enzyme
Also known as:
- Spanish: Proteinasa K, Enzima digestión de proteÃnas, Proteasa K
- French: Protéinase K, Enzyme digestion des protéines, Protéase K
- German: Proteinase K, Proteinabbauenzym, Protease K
- Italian: Proteinasi K, Enzima digestione proteine, Proteasi K
FAQs
Q: What is the main difference with Proteinase K lyophilized powder?
A: The solution is ready-to-use, while the powder requires reconstitution.
Q: Is it resistant to SDS and EDTA?
A: Yes, it maintains activity in denaturing conditions.
Q: Can it be used for both DNA and RNA extraction?
A: Yes, it is widely used in genomic and transcriptomic workflows.
1 review for Proteinase K Solution
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David Anderson –
We use this Proteinase K solution regularly during genomic DNA extraction workflows in our lab. The liquid format is convenient since it can be added directly without preparing fresh stocks, which simplifies the protocol. It performs well even in buffers containing detergents, which is useful during lysis steps.