AMV Reverse Transcriptase
High-quality AMV Reverse Transcriptase for cDNA synthesis and RNA analysis.
AMV Reverse Transcriptase is an RNA-dependent DNA polymerase derived from Avian Myeloblastosis Virus and produced in E. coli. It synthesizes the first-strand cDNA from RNA templates and also acts on DNA or RNA:DNA hybrids.
The enzyme combines multiple activities: RNA- and DNA-directed DNA polymerase, RNase H, DNA-RNA unwinding, and Mn²⁺-dependent endonuclease. With high purity (>90%), free of RNase and DNase, it ensures reproducible results in reverse transcription, cDNA synthesis, and RNA sequencing workflows.
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Price: 296.70 €
Detailed information:
Advantages & Features
- Concentration: 10 U/µL
- Full Solution: includes Reaction Buffer 5x.
- High quality: free of RNase, and DNase (exo- and endonuclease) activities.
- Multiple enzymatic activities for robust performance.
Specifications
Unit definition:
One unit is the amount of enzyme required to catalyze the transfer of 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37 °C, using poly(A) oligo dT as a template primer.
Includes
– 1,000 U AMV Reverse Transcriptase (10 U/μL)
– Reaction Buffer 5x
Download documentation
Datasheet MSDSApplications
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RT-PCR.
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First-strand cDNA synthesis.
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RNA sequencing workflows.
Tables & Figures
Quality Control
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Free of nucleases (endo-, exonuclease, RNase).
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Purity >90% confirmed by SDS-PAGE.
Advice
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Use poly(A) oligo dT primers for optimal cDNA yield.
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Keep enzyme on ice during setup.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
- Zhu, M., Zhang, C., Zhou, P., Chen, S., & Zheng, H. (2022). LncRNA CASC15 upregulates cyclin D1 by downregulating miR-365 in laryngeal squamous cell carcinoma to promote cell proliferation. Journal of Otolaryngology-Head & Neck Surgery, 51(1), 1-7.
- Zhang, Z., Ao, P., Han, H., Zhang, Q., Chen, Y., Han, J., … & Zhuo, D. (2020). LncRNA PLAC2 upregulates miR-663 to downregulate TGF-β1 and suppress bladder cancer cell migration and invasion. BMC Urology, 20(1), 1-7.
- Jiang, X., Wang, J., & Fang, L. (2020). LncRNA WT1-AS over-expression inhibits non-small cell lung cancer cell stemness by down-regulating TGF-β1. BMC Pulmonary Medicine, 20(1), 1-6.
- Yang, L., Liu, G., Xiao, S., Wang, L., Liu, X., Tan, Q., & Li, Z. (2020). Long Noncoding MT1JP Enhanced the Inhibitory Effects of miR-646 on FGF2 in Osteosarcoma. Cancer Biotherapy & Radiopharmaceuticals.
- Liu, Y., Li, M., Yu, H., & Piao, H. (2019). LncRNA SRA1 is down-regulated in HPV-negative cervical squamous cell carcinoma and regulates cancer cell behaviors. Bioscience reports, 39(8), BSR20191226.
- Zhong, Y., Li, Y., Song, T., & Zhang, D. (2019). MiR-718 mediates the indirect interaction between lncRNA SEMA3B-AS1 and PTEN to regulate the proliferation of hepatocellular carcinoma cells. Physiological genomics.
- Zhang, P., Liu, Y., Li, C., Zhang, L., Liu, Q., & Jiang, T. LncRNA PAPAS promotes oral squamous cell carcinoma by upregulating transforming growth factor‐β1. Journal of Cellular Biochemistry.
- Yang, S., Cai, H., Hu, B., & Tu, J. (2019). LncRNA SAMMSON negatively regulates miR-9-3p in hepatocellular carcinoma cells and has prognostic values. Bioscience Reports, BSR20190615.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q: What is the concentration?
A: 10 U/µL (1000 U supplied).
Q: Does it have RNase H activity?
A: Yes, intrinsic RNase H activity is present.
Q: What applications is it best suited for?
A: cDNA synthesis, RT-PCR, RNA sequencing.
Reviews