One-Step RT PCR Kit
Convenient one-step RT-PCR kit combining cDNA synthesis and PCR amplification in a single reaction.
The One-Step RT PCR Kit enables highly efficient cDNA synthesis and PCR amplification in a single tube, streamlining workflows and reducing contamination risks. The kit includes a thermostable reverse transcriptase with RNase inhibitor for reliable cDNA yields, combined with a hot-start Taq DNA polymerase for specific and sensitive amplification.
Validated for GC-rich and complex RNA templates, this kit is ideal for gene expression studies, RNA virus detection, and diagnostic research. It ensures reproducibility, nuclease-free quality, and consistent results across batches.
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Price: 283.00 €
Detailed information:
Advantages & Features
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Single-tube workflow: minimizes handling and contamination.
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High specificity & sensitivity.
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Hot-start Taq DNA polymerase with optimized dNTPs.
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Reliable: consistent results with GC-rich templates.
Specifications
Includes
– 2 x 1.25 mL One-Step PCR Mix (2x)
– 2 x 125 μL RT Mix
– 2 mL Water (RNase-free)
Download documentation
Datasheet MSDSApplications
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One-step RT-PCR.
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Gene expression analysis.
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RNA virus detection.
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Amplification of GC-rich and complex templates.
Tables & Figures
Quality Control
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Each lot tested with eukaryotic total RNA.
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Verified free of DNase, RNase, and protease contamination.
Advice
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Thaw and mix reagents gently before use.
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Avoid repeated freeze-thaw cycles.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
One-step RT-PCR kit, Reverse transcription PCR kit, RT-PCR master mix, RNA to DNA amplification kit, RT-PCR reaction mix
Also known as:
- Spanish: Kit RT-PCR en un paso, Kit de retrotranscripción PCR, Mezcla RT-PCR
- French: Kit RT-PCR en une étape, Kit de transcription inverse PCR, Mélange RT-PCR
- German: One-Step RT-PCR Kit, Reverse-Transkriptions-PCR Kit, RT-PCR-Mix
- Italian: Kit RT-PCR one-step, Kit retrotrascrizione PCR, Miscela RT-PCR
FAQs
Q: What makes this kit different from two-step RT-PCR?
A: It combines cDNA synthesis and PCR in one tube, saving time and reducing contamination risk.
Q: Is it suitable for GC-rich templates?
A: Yes, specifically optimized for challenging RNA templates.
Q: What is included?
A: PCR Mix, RT Mix, and RNase-free water.
Reviews