First Strand cDNA Synthesis Kit

Includes for 50 rxn:
– 50 μL Reverse Transcriptase (200U/µL)
– 500 μL cDNA Synthesis Buffer (5x)
– 50 μL dNTP Mix (10 mM)
– 50 μL Hexamer Primer (200ng/µL)
– 50 μL Oligo (dT)20(50µM)
– 25 μL RNase Inhibitor (50U/µL)
– 1 mL Nuclease-free Water
– 250 µL  DTT (0.1 M)

• RT-PCR and qPCR assays.

• Gene expression analysis.

• Cloning of cDNA sequences.

• Transcriptome and RNA profiling studies.

Each batch is validated for reverse transcription efficiency, absence of RNase/DNase contamination, and performance in qPCR assays.

• Use high-quality RNA to maximize cDNA yield.

• Mix oligo(dT) and random primers for broad transcript coverage.

• Store reagents in aliquots to avoid repeated freeze-thaw cycles.

• Shipment at Gel Pack.
• Storage: Upon receipt of the kit, immediately store the components at -20 °C in a freezer without a defrost cycle. It is recommended to reduce freeze-thaw cycles as less as possible.

• Kamau, M., Ergunay, K., Bourke, B. P., Mutura, J., Lebunge, R., Ochieng, G., … & Linton, Y. M. (2025). Potential spillover investigated by metagenome sequencing in Laikipia, Kenya reveals tick-borne pathogens and a novel bunyavirus. One Health, 101226.
• Todorova, M. N., Savova, M. S., Mihaylova, L. V., & Georgiev, M. I. (2023). Icariin Improves Stress Resistance and Extends Lifespan in Caenorhabditis elegans through hsf-1 and daf-2-Driven Hormesis. International Journal of Molecular Sciences, 25(1), 352.
• Saleem, M. A., Mustafa, M. S., & Mustafa, M. (2024). Promoter Hypermethylation of the BRCA1 Gene as a Novel Biomarker for Prostate Cancer. Cureus, 16(8).
• Amirova, K., Dimitrova, P., Koycheva, I., Balcheva-Sivenova, J., & Georgiev, M. I. Modulation of Nrf2 expression in human neutrophils by Ballota nigra extract.
• Savova, M. S., Todorova, M. N., Apostolov, A. G., Yahubyan, G. T., & Georgiev, M. I. (2022). Betulinic acid counteracts the lipid accumulation in Caenorhabditis elegans by modulation of nhr-49 expression. Biomedicine & Pharmacotherapy, 156, 113862.
• Koycheva, I. K., Vasileva, L. V., Amirova, K. M., Marchev, A. S., Balcheva-Sivenova, Z. P., & Georgiev, M. I. (2021). Biotechnologically produced Lavandula angustifolia Mill. extract rich in rosmarinic acid resolves psoriasis-related inflammation through Janus kinase/signal transducer and activator of transcription signaling. Frontiers in pharmacology, 940.
• Mladenova, S. G., Savova, M. S., Marchev, A. S., Ferrante, C., Orlando, G., Wabitsch, M., & Georgiev, M. I. (2022). Anti-adipogenic activity of maackiain and ononin is mediated via inhibition of PPARγ in human adipocytes. Biomedicine & Pharmacotherapy, 149, 112908.
• Mladenova, Saveta G., et al. “Anti-adipogenic Effect of Alchemilla monticola is Mediated via PI3K/AKT Signaling Inhibition in Human Adipocytes.“ Frontiers in Pharmacology: 2179.
• Savova, Martina S., et al. “Ziziphus jujuba Mill. leaf extract restrains adipogenesis by targeting PI3K/AKT signaling pathway.” Biomedicine & Pharmacotherapy 141 (2021): 111934.
• Lago-Docampo, M., Tenorio, J., Hernández-González, I., Pérez-Olivares, C., Escribano-Subías, P., Pousada, G., … & Valverde, D. (2020). Characterization of rare ABCC8 variants identified in Spanish pulmonary arterial hypertension patients. Scientific Reports, 10(1), 1-13.
• Petkova, Mariana, et al. “MICROBIOLOGICAL AND PHYSICOCHEMICAL CHARACTERIZATION OF TRADITIONAL BULGARIAN SOURDOUGHS AND SCREENING OF LACTIC ACID BACTERIA FOR AMYLOLYTIC ACTIVITY.” Journal of Chemical Technology and Metallurgy 55.5 (2020): 921-934.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q1: What is the minimum RNA input required?
The kit is optimized for a broad range, from low-input RNA to higher concentrations.

Q2: Can I use both total RNA and mRNA?
Yes, it is compatible with both sample types.

Q3: What downstream applications are supported?
It is validated for RT-PCR, qPCR, cloning, and transcriptome studies.