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Inorganic Pyrophosphatase
High-Quality (GMP grade) Inorganic Pyrophosphatase for In Vitro Transcription Reactions.
Inorganic Pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate into orthophosphate, driving biosynthetic reactions forward. It is commonly used to enhance yields in transcription and enzymatic assays.
The enzyme offers high stability and consistent activity, ensuring reliable reaction performance in molecular biology applications.
Our IPP enzyme can be applied across diverse fields such as the production of therapeutic mRNA and DNA, diagnostics and any kind of nucleotide incorporation reactions, among others.
To know more about others IVT enzymes available visit this page.
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Price: 172.90 €
Detailed information:
Advantages & Features
- Optimizes in vitro transcription (IVT): Efficiently removes inorganic pyrophosphate (PPi), a byproduct that inhibits RNA polymerase activity, significantly enhancing RNA yield.
- High purity: ≥ 95% purity confirmed by SDS-PAGE; free from detectable endonuclease, exonuclease, RNase, and alkaline phosphatase activity amongs others host cell proteins and DNA.
- Safety: Low endotoxin (≤ 0.5 EU/U) suitable for sensitive RNA workflows. Animal-origin-free, β-lactam-free manufacturing
- Recombinant origin in E. coli: Produced without animal-derived materials or β-lactam antibiotics, minimizing regulatory and biosafety concerns.
- Versatile applications: Suitable for therapeutic mRNA synthesis, DNA production, molecular diagnostics, and any nucleotide-incorporation reaction.
- Ready-to-use 0.1 U/µL stock: matches common IVT protocols
- Produced under ISO13485 & cGMP principles
Specifications
Protein information
Predicted MW: Homohexamer with 19,71 kDa subunits
Purity: ≥ 95% by SDS-PAGE
Buffer: 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2, 2 mM PPi
Storage: 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH=8
Biological activity: One unit is the amount of enzyme that will generate 1 µmol ofphosphate per minute from PPi under standard reaction conditions (10 minutes reaction at 25°C) in reaction buffer.
Gene Information
Synonyms: PPAse
Official Symbol: E-PPAse
Specie: Escherichia coli
GenBank accession number: NC_000913 (4449125-4449652)
NCBI Protein RefSeq: NP_418647
Protein Family: Bacterial and archae inorganic pyrophosphatases, hydrolases
Includes
– 50 U Inorganic Pyrophosphatase (0,1 U/µL)
Download documentation
Datasheet MSDS BrochureApplications
- High-yield mRNA & gRNA in vitro transcription
- DNA replication & labelling workflows
- Diagnostic assay and kit manufacturing
- Any PPi-generating polymerase reaction
Tables & Figures
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Quality Control
Quality Tests includes:
- Appearance: Clear, colorless solution
- pH: 8 ± 0,3
- Purity & Identity by SDS-PAGE: ≥ 95 % and 19 ± 3 kDa
- Activity: > 100 U/mL
- Endonuclease Activity (nicking): No detectable
- Exonuclease Activity: No detectable
- RNAse Activity: No detectable
- Alkaline Phosphatase Activity: No detectable
- Residual genomic DNA: < 350 pg/ml
- Endotoxins: ≤ 0,5 EU/U
- Bioburden: ≤ 2 CFU/10U
Advice
Avoid exposure to constant temperature changes.
Storage & Shipping
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
Inorganic pyrophosphatase enzyme, Pyrophosphate phosphohydrolase, PPase enzyme, Pyrophosphate hydrolysis enzyme, Recombinant inorganic pyrophosphatase
Also known as:
- Spanish: Pirofosfatasa inorgánica, Enzima PPasa, Hidrolasa de pirofosfato
- French: Pyrophosphatase inorganique, Enzyme PPase, Hydrolase du pyrophosphate
- German: Anorganische Pyrophosphatase, PPase-Enzym, Pyrophosphat-Hydrolase
- Italian: Pirofosfatasi inorganica, Enzima PPasi, Idrolasi del pirofosfato
FAQs
- What does “GMP-grade” mean for an RUO enzyme?
The enzyme is labeled for Research Use Only but manufactured under a controlled quality system, applying relevant GMP and ICH quality principles for traceability, change control and lot release. “GMP-grade” describes the manufacturing controls applied; it does not imply approval as a medicinal product or API. - Which certificates are supplied with every lot?
• Certificate of Analysis (activity, purity, nuclease tests, endotoxin)
• Certificate of Origin & Animal-Free statement - Does IPP work with T7 and/or SP6 polymerases?
Yes. It hydrolyses PPi in any DNA-dependent RNA-polymerase reaction. Recommended dose will be in the range of 0.005–0.01 U per µL of final IVT mix. - What is the shelf life and storage?
24 months at −20 °C. Avoid freeze-thaw cycles; aliquot if you open the vial frequently. - What’s the endotoxin specification?
≤ 0.5 EU per unit, tighter than the spec disclosed for most competing PPases.
Elena Petrova –
We added this enzyme to our in vitro transcription workflow after reviewing the recommended protocol. The reagent is easy to incorporate into reaction preparation and does not complicate the overall process. It has worked well within our routine RNA production experiments.
Michael Turner –
Excelent alternative to NEB and Thermo, better quality and price