SP6 Polymerase
High-yield SP6 RNA Polymerase (20,000 U/mL) for in vitro transcription of RNA from DNA templates containing the SP6 promoter. Optimized for high purity, reproducibility, and robust performance.
SP6 RNA Polymerase (20,000 U/mL) is an RNA polymerase enzyme that catalyzes the synthesis of RNA in vitro using DNA templates containing an SP6 promoter. It is widely used in transcription studies, RNA probe preparation, and mRNA synthesis.
The enzyme offers high activity and purity, ensuring efficient transcription and consistent yields in molecular biology applications.
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Price: 67.00 €
Detailed information:
Advantages & Features
- High activity: reliable transcription efficiency.
- Free of RNase contamination.
- Supplied at 20,000 U/mL for convenient use.
- Produces RNA up to several kilobases in length.
- Suitable for radioactive or non-radioactive labeling.
- Flexible Quality Systems that cover from RUO to cGMP.
- High-Purity: ≥ 95% determined by SDS.
Specifications
Protein information
Predicted MW: 98,55 kDa.
Purity: ≥ 95% by SDS-PAGE.
Storage Buffer: 50mM Tris-HCl buffer (pH 7.9 at 25°C), 100mM NaCl, 20 mM β-ME, 1mM EDTA, 0.1% (v/v) Triton® X-100, 50% (v/v) glycerol.
Biological activity: One unit is defined as the amount of enzyme required to catalyze the incorporation of 5 nmol of CTP into acid-insoluble product in 60 minutes at 37 °C.
Gene Information
Official Symbol: SP6 RNAP.
Specie SP6: Bacteriophage.
GenBank accession number: AY288927.2
NCBI Protein RefSeq: P06221.
Protein Family: Phage and mitochondrial DNA-dependent RNA Polymerase.
Includes
– 2,000 U SP6 RNA Polymerase (20U/µL)
Download documentation
Datasheet MSDSApplications
- In vitro mRNA synthesis (DNA sequence to transcript must be cloned downstream SP6 promoter)
- Synthesis of RNA probes for Northern/Southern blotting
- Production of labeled RNAs for in situ hybridization
- Template generation for cell-free translation systems
- Functional RNA studies (ribozymes, aptamers, etc.)
- CRISPR guide RNA preparation
Tables & Figures
Quality Control
Quality Tests for Research Use Only version includes: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.
Advice
Use RNase-free water, tips and consumables when working with RNA.
Always store enzyme on ice during use.
Storage, Shipping & Guarantee
- Shipped in:Â Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
To be informed about our Safety Staments for GMP Products, please contact us.
Customers Review
Synonym(s)
SP6 RNA polymerase, DNA-dependent RNA polymerase SP6, In vitro transcription enzyme SP6, SP6 transcription enzyme, RNA synthesis enzyme SP6
Also known as:
- Spanish: ARN polimerasa SP6, Enzima de transcripción SP6, Polimerasa dependiente de ADN SP6
- French: ARN polymérase SP6, Enzyme de transcription SP6, Polymérase dépendante de l’ADN SP6
- German: SP6-RNA-Polymerase, Transkriptionsenzym SP6, DNA-abhängige RNA-Polymerase SP6
- Italian: RNA polimerasi SP6, Enzima di trascrizione SP6, Polimerasi RNA dipendente da DNA SP6
FAQs
Q1: Can SP6 Polymerase transcribe any DNA template?
A1: No, the DNA must contain an SP6 promoter upstream of the target sequence.
Q2: What is the maximum transcript length?
A2: Several kilobases, depending on template quality and conditions.
Q3: Is the enzyme RNase-free?
A3: Yes, each batch is rigorously tested for RNase contamination.
Klaus Berger –
Wir verwenden die SP6 RNA Polymerase regelmaessig fuer die Synthese von RNA-Sonden zur Hybridisierung. Die Enzymaktivitaet ist zuverlaessig und liefert konsistente Ausbeuten bei der Transkription aus SP6-Promotor-haltigen Vektoren. Die RNase-Freiheit ist besonders wichtig bei uns, da wir empfindliche Proben verarbeiten. Fuer routinemaessige in-vitro-Transkriptionsexperimente eine gute Wahl.