CVX™ SP6 Transcription Kit
High-yield SP6 RNA transcription kit for efficient in vitro RNA synthesis.
CVX™ SP6 Transcription Kit provides a robust and reliable system for in vitro RNA synthesis using SP6 RNA polymerase. The optimized enzyme and buffer formulation deliver high yields of full-length RNA transcripts with precise sequence fidelity, suitable for a wide range of molecular biology and RNA-based research applications.
This ready-to-use kit simplifies transcription setup and ensures consistent performance across templates containing an SP6 promoter. It is ideal for generating RNA for probe labeling, translation studies, and synthetic RNA production in both research and development workflows.
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Price: 324.00 €
Detailed information:
Advantages & Features
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High transcription efficiency: Produces large amounts of full-length RNA.
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Optimized SP6 enzyme mix: Ensures strong promoter recognition and stability.
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All-in-one formulation: Reduces pipetting errors and setup time.
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Reproducible yields: Provides batch-to-batch consistency and purity.
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Flexible template use: Compatible with linearized plasmid or PCR DNA containing an SP6 promoter.
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Ideal for labeling workflows: Works seamlessly with CVX™ RNA Labeling Kits.
Specifications
Includes
– 3 x 40 μl SP6 RNA Polymerase mix (Include RNase inhibitor)
– 200 μl SP6 Reaction Buffer 10 x (HEPES-based)
– 100 μl ATP (100 mM)
– 100 μl GTP (100 mM)
– 100 μl CTP (100 mM)
– 100 μl UTP (100 mM)
– 100 μl DTT (100 mM)
– 10 µl Control template 1 (200 ng/µl)
– 10 µl Control template 2 (200 ng/µl)
– 1.2 ml Nuclease-Free Water
Download documentation
Datasheet MSDSApplications
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In vitro RNA transcription and synthesis.
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Probe and antisense RNA generation.
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RNA standard preparation for qPCR.
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Translation and hybridization assays.
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Synthetic RNA production for molecular biology workflows.
Tables & Figures
Quality Control
- Each lot is tested for transcription yield, RNA integrity, and RNase-free quality to ensure reproducibility and performance consistency.
Advice
- Use high-purity, linearized DNA as a transcription template.
- Avoid repeated freeze–thaw cycles of reagents.
- Maintain RNase-free conditions throughout the workflow.
- Store small aliquots for long-term enzyme stability.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What templates are compatible with this kit?
Linearized plasmid or PCR-amplified DNA containing an SP6 promoter sequence.
Q2: Can this kit be used with labeling reagents?
Yes. It is fully compatible with CVX™ RNA Labeling Kits (Biotin, DIG, or Fluorescein).
Q3: What is the typical RNA yield per reaction?
Up to 100 µg of full-length RNA per 20 µL transcription reaction.
