T7 Digoxigenin RNA Labeling Kit
High-efficiency in vitro transcription kit for Digoxigenin-labeled RNA probe generation.
T7 Digoxigenin RNA Labeling Kit provides a robust and efficient solution for in vitro transcription and DIG-labeling of RNA using T7 RNA polymerase. The system incorporates Digoxigenin-UTP directly into RNA transcripts during synthesis, producing stable, non-radioactive RNA probes for hybridization and detection assays.
This optimized single-step reaction delivers reproducible labeling efficiency and excellent transcription yields. Ideal for Northern blotting, in situ hybridization, and RNA–protein interaction studies, it enables sensitive and specific probe detection using anti-DIG conjugates.
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Detailed information:
Advantages & Features
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Integrated labeling system: Combines transcription and DIG-labeling in one step.
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High sensitivity: Generates DIG-labeled RNA for precise, non-radioactive detection.
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Optimized T7 enzyme mix: Ensures efficient DIG-UTP incorporation.
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Reproducible yields: Consistent labeling and transcription performance.
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Safe and convenient: Non-radioactive method for hybridization assays.
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Broad compatibility: Works with T7 promoter-based templates (plasmid or PCR).
Specifications
Includes
– 2x 40 μl T7 RNA Polymerase mix
– 200 μl T7 10X Reaction Buffer
– 100 μl ATP (100 mM)
– 100 μl GTP (100 mM)
– 100 μl CTP (100 mM)
– 100 μl UTP (100 mM)
– 25 μl DIG-11-UTP (10mM)
– 150 μl DTT (100 mM)
– 10 µl Control template 1 (200 ng/µl)
– 1.2 ml Nuclease-Free Water
Download documentation
Datasheet MSDSApplications
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DIG-labeled RNA probe synthesis.
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In situ hybridization (ISH).
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Northern and dot blot analysis.
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RNA localization and detection studies.
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RNA–protein binding assays.
Tables & Figures
Quality Control
- Each lot is tested for labeling efficiency, transcription yield, and RNase-free integrity to guarantee consistent DIG incorporation and hybridization performance.
Advice
- Use high-quality, linearized templates with a T7 promoter.
- Protect all reagents from RNase contamination.
- Verify labeling by dot blot analysis using anti-DIG antibodies.
- Store reagents in small aliquots to avoid repeated freeze–thaw cycles.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What labeling molecule is incorporated?
Digoxigenin-UTP is incorporated during transcription for non-radioactive RNA probe labeling.
Q2: Is this kit compatible with other polymerases?
Yes. It can be used with templates designed for CVX™ SP6 or T3 RNA Synthesis Kits.
Q3: How can labeling efficiency be verified?
Detection can be confirmed by dot blot or hybridization using anti-DIG–AP or anti-DIG–HRP conjugates.
