CVX™ T7 ARCA mRNA Synthesis Kit
High-efficiency ARCA-capped mRNA synthesis kit for in vitro transcription using T7 RNA polymerase.
CVX™ T7 ARCA mRNA Synthesis Kit provides a high-efficiency system for capped mRNA synthesis using Anti-Reverse Cap Analog (ARCA). The optimized T7-based transcription mix produces full-length, capped RNA transcripts with superior translational efficiency and stability, ideal for mRNA functional studies and vaccine research.
This ready-to-use formulation ensures consistent yield, precise capping, and reduced 5′-end heterogeneity. The system is fully compatible with linearized DNA templates containing a T7 promoter, enabling reproducible mRNA synthesis for transfection, translation, or downstream modification.
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Detailed information:
Advantages & Features
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High capping efficiency: Incorporates ARCA at the 5′-end for functional mRNA synthesis.
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Superior translational performance: Generates mRNA with enhanced stability and expression.
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Optimized T7 transcription mix: Ensures full-length RNA transcripts and reduced abortive products.
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Reproducible yield: Delivers consistent mRNA quality across batches.
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Ready-to-use reagents: Simplifies setup and minimizes variability.
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Broad compatibility: Works with linearized plasmid or PCR templates containing a T7 promoter.
Specifications
Includes
– 3 x 40 μl T7 RNA Polymerase mix (include RNase Inhibitor)
– 200 μl T7 Reaction Buffer 10x (HEPES-based)
– 100 μl ATP (100 mM)
– 100 μl GTP (100 mM)
– 100 μl CTP (100 mM)
– 100 μl UTP (100 mM)
– 100 μl DTT (100 mM)
– 10 µl ARCA Solution (100mM)
– 10 µl T7 Control template 2 (200 ng/µl)
– 1.2 ml Nuclease-Free Water
Download documentation
Datasheet MSDSApplications
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Capped mRNA synthesis for translation assays.
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mRNA vaccine and therapeutic research.
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In vitro translation systems.
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Functional mRNA and reporter studies.
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CRISPR guide RNA and synthetic biology applications.
Tables & Figures
Quality Control
- Each lot is tested for capping efficiency, RNA yield, and template integrity to ensure reproducible and RNase-free mRNA synthesis.
Advice
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Use high-purity linearized DNA as the transcription template.
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Avoid repeated freeze–thaw cycles of reagents.
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Keep all components on ice during setup.
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Verify mRNA quality via denaturing agarose gel or spectrophotometry.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What is ARCA and why is it important?
ARCA (Anti-Reverse Cap Analog) ensures proper orientation of the 5′-cap, enhancing translation and RNA stability.
Q2: Can this kit be used to synthesize long mRNA sequences?
Yes. The system efficiently transcribes RNAs up to several kilobases with high integrity.
Q3: Is the resulting mRNA suitable for transfection experiments?
Yes. It produces biologically active, capped mRNA ideal for cell-based and in vitro translation assays.
