T7 Biotin16 RNA Labeling Kit
High-efficiency in vitro transcription kit for RNA labeling with Biotin-16-UTP
T7 Biotin-16 RNA Labeling Kit provides a fast, reliable, and efficient system for in vitro transcription and biotin labeling of RNA. The kit incorporates Biotin-16-UTP during transcription using T7 RNA polymerase, generating high-quality, biotinylated RNA suitable for downstream hybridization and detection assays.
Its optimized formulation ensures consistent labeling efficiency and yield, eliminating the need for post-synthesis modification steps. Ideal for probe generation, microarray applications, Northern blotting, and RNA–protein interaction studies, it enables high-sensitivity detection with strong signal integrity.
Have questions? Contact us
Detailed information:
Advantages & Features
-
Integrated labeling system: Combines transcription and biotin labeling in a single step.
-
High signal intensity: Produces biotin-labeled RNA with excellent hybridization properties.
-
Optimized T7 enzyme mix: Ensures efficient incorporation of Biotin-16-UTP.
-
Reproducible performance: Delivers consistent yield and labeling ratio.
-
Ready-to-use formulation: Reduces hands-on preparation and variability.
-
Broad compatibility: Works with T7 promoter-based templates (linearized plasmids or PCR DNA).
Specifications
Includes
– 3 x 40 μl T7 RNA Polymerase mix (include RNase Inhibitor)
– 200 μl T7 Reaction Buffer 10x (HEPES-based)
– 100 μl ATP (100 mM)
– 100 μl GTP (100 mM)
– 100 μl CTP (100 mM)
– 100 μl UTP (100 mM)
– 100 μl DTT (100 mM)
– 30 µl Biotin-16-UTP (10mM)
– 10 µl T7 Control template 1 (200 ng/µl)
– 1.2 ml Nuclease-Free Water
Download documentation
Datasheet MSDSApplications
-
Biotin-labeled RNA probe synthesis.
-
Microarray and blot hybridization.
-
RNA–protein binding and pull-down assays.
-
RNA tracking and localization studies.
-
Detection assays using streptavidin conjugates.
Tables & Figures
Quality Control
- Each lot is tested for transcription efficiency, labeling incorporation rate, and RNase-free integrity to ensure reproducible, high-quality RNA synthesis.
Advice
-
Use linearized plasmid or PCR-generated templates containing a T7 promoter.
-
Protect reactions from RNase contamination.
-
Verify labeling efficiency using streptavidin-based detection.
-
Store enzyme mixes and nucleotides in aliquots to prevent freeze–thaw cycles.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What type of biotin label is incorporated?
Biotin-16-UTP is integrated during transcription, enabling direct detection with streptavidin conjugates.
Q2: Is it compatible with other RNA synthesis kits?
Yes. It works seamlessly with templates designed for CVX™ T7, SP6, or T3 RNA Synthesis Kits.
Q3: Can I use the RNA for hybridization assays?
Yes. The labeled RNA is ideal for microarray, Northern blot, and RNA–protein binding experiments.
Reviews