CVX™ T7 RNA Synthesis Kit
High-yield T7 RNA transcription kit for in vitro RNA synthesis from DNA templates.
CVX™ T7 RNA Synthesis Kit enables rapid and efficient in vitro transcription of high-quality RNA using T7 RNA polymerase and an optimized buffer system. The formulation ensures robust RNA synthesis from linearized DNA templates, providing excellent yields suitable for downstream molecular biology and RNA-based research applications.
This all-in-one kit contains all required components for transcription reactions, minimizing setup time and improving reproducibility. It is ideal for generating RNA for probe labeling, translation assays, or template production in a variety of molecular workflows.
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Detailed information:
Advantages & Features
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High RNA yield: Produces large amounts of full-length RNA transcripts.
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Optimized T7 enzyme system: Delivers strong promoter recognition and transcription efficiency.
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All-in-one formulation: Includes all necessary reagents for reliable synthesis.
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Reproducible performance: Provides batch-to-batch consistency and purity.
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Flexible template compatibility: Works with linearized plasmids or PCR products.
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Time-saving workflow: Reduces hands-on preparation and setup errors.
Specifications
| Parameter | Specification |
|---|---|
| Enzyme | T7 RNA Polymerase |
| Reaction format | 20 µL standard reaction |
| Typical yield | > 100 µg RNA per reaction |
| Reaction time | 1–2 h |
| Compatible templates | Linearized plasmid or PCR DNA |
| Nuclease contamination | None detected |
Includes
– 3 x 40 μl T7 RNA Polymerase mix (Include RNase Inhibitor)
– 200 μl T7 Reaction Buffer 10x (HEPES-based)
– 100 μl ATP (100 mM)
– 100 μl GTP (100 mM)
– 100 μl CTP (100 mM)
– 100 μl UTP (100 mM)
– 100 μl DTT (100 mM)
– 10 µl Control template 1 (200 ng/µl)
– 10 µl Control template 2 (200 ng/µl)
– 1.2 ml Nuclease-Free Water
Download documentation
Datasheet MSDSApplications
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In vitro transcription assays.
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Probe and antisense RNA synthesis.
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In vitro translation systems.
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RNA standard or template production.
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Functional RNA and CRISPR guide RNA preparation.
Tables & Figures
Quality Control
- Each batch is tested for transcription efficiency, template integrity, and absence of RNase contamination to ensure reproducible RNA yields and reliability.
Advice
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Use high-purity, linearized DNA as the transcription template.
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Keep reagents on ice during reaction setup.
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Treat surfaces and tools with RNase-free precautions.
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Store aliquots to avoid freeze–thaw cycles.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What is the typical RNA yield per reaction?
Yields vary with template length but typically exceed 100 µg per 20 µL reaction.
Q2: Can the synthesized RNA be used directly for translation?
Yes. The RNA is suitable for in vitro translation, labeling, and hybridization experiments.
Q3: Which DNA templates are compatible?
Linearized plasmid DNA or PCR-amplified templates containing a T7 promoter sequence.
