Vaccinia Capping Enzyme
High-purity Vaccinia Capping Enzyme for efficient 5′ RNA capping in in vitro transcription workflows. Ideal for mRNA synthesis, vaccines, and gene therapy research.
Vaccinia Capping Enzyme catalyzes the addition of a 5′ cap structure to RNA transcripts, improving stability and translation efficiency. It is used in in vitro transcription systems to produce capped mRNA.
The enzyme ensures high activity and reliable performance, making it suitable for mRNA synthesis, translation studies, and gene expression research.
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Detailed information:
Advantages & Features
- Convenient solution that includes all components required for successful Enzymatic capping System
- ≥95% purity verified by SDS-PAGE
- High capping efficiency for superior RNA translation
- Recombinant production ensures batch-to-batch consistency
- Compatible with a wide range of RNA polymerases
Specifications
Unit definition: One unit of Vaccinia Capping Enzyme is defined as the amount of enzyme required to incorporate 10 pmol of (α32P) GTP into an 80 nt transcript in 1 hour at 37°C.
Includes
– 400 U Vaccinia Capping Enzyme (10 U/µL)
– Reaction Buffer 10x (500 mM Tris-HCl pH=8,0; 10 mM MgCl2; 50 mM KCl; 10 mM DTT)
– GTP (10 mM)
– S-adenosylmethionine (SAM) 40 mM
Download documentation
Datasheet MSDSApplications
- Labeling 5´ end of mRNA.
- mRNA vaccine development
- Gene therapy and RNA therapeutics
- In vitro transcription and synthetic biology
- Functional studies on RNA processing
Tables & Figures
Quality Control
Quality Tests for Research Use Only version includes: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.
Advice
For optimal results, use fresh RNA transcripts and maintain RNase-free conditions during reactions. Avoid multiple freeze–thaw cycles.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
To be informed about our Safety Staments for GMP Products, please contact us.
Customers Review
FAQs
Q1: Is this enzyme compatible with T7 RNA polymerase?
A: Yes, it is optimized for use with T7 and other commonly used RNA polymerases.
Q2. Can it be used in large-scale mRNA production?
A: Yes, the enzyme is suitable for both small-scale and scalable workflows.
Reviews