2-O-Methyl Transferase
Cap-1 formation enzyme (Vaccinia VP39) for IVT RNA: converts Cap-0 to Cap-1 using SAM; boosts translation and reduces innate immune activation. 2,000 U (EZ0040).
High-quality recombinant mRNA Cap 2′-O-Methyltransferase from Vaccinia virus, produced in E. coli, for efficient Cap-1 formation.
The mRNA Cap 2′-O-Methyltransferase transfers a methyl group to the 2′-O position of the first nucleotide adjacent to the m7G cap (Cap-0), converting capped RNA into a Cap-1 structure using S-adenosyl-L-methionine (SAM) as methyl donor. Cap-1 enhances translation efficiency and helps IVT mRNA evade innate immune sensing in many cell types—key for transfection, microinjection and therapeutic mRNA workflows.
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Detailed information:
Advantages & Features
- Reliable Cap-1 formation from Cap-0 RNA using SAM.
- Improved expression: Cap-1 supports higher translation and protein yield.
- Lower innate immune sensing vs Cap-0 in many systems.
- Recombinant production in E. coli; ≥95% purity by SDS-PAGE.
- Complete kit components (enzyme, SAM, 10× buffer) for immediate use.
- Extensivelly quality controlled: Endo/Exonucleases/RNAses free.
Specifications
Protein information
Source: Vaccinia virus VP39 (recombinant, E. coli)
Predicted MW: 39 kDa
Purity: ≥ 95% (SDS-polyacrylamide gels with Coomassie).
Buffer Storage: 100 mM NaCl, 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1 % Triton®X-100.
Biological activity: One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt long capped RNA transcript in 1 hour at 37°C.
Includes
– 2,000 U mRNA Cap 2′-O-Methyltransferase (50 U/µl)
– S-adenosylmethionine (SAM)
– Capping Buffer (10x)
Download documentation
Datasheet MSDSApplications
- Convert Cap-0 IVT RNA to Cap-1 to enhance translation.
- Reduce innate immune activation in non-immunotherapy mRNA uses.
- Downstream use in transfection, microinjection, and in vitro translation assays.
Tables & Figures
Quality Control
Quality Tests for Research Use Only version includes: Activity, SDS-PAGE/purity, DNase, RNase and endonuclease.
Advice
- Use RNase-free plastics and reagents; avoid freeze–thaw cycles.
- Keep the enzyme on ice during setup; pre-warm RNA to 37 °C for the reaction.
- For Cap-0 substrates, ensure prior capping (e.g., Vaccinia Capping Enzyme) before 2′-O methylation.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1. What substrate does the enzyme require?
Capped RNA with m7GpppN (Cap-0) at the 5′ end; it does not modify uncapped RNA.
Q2. Do I need Vaccinia Capping Enzyme before using this product?
Yes, if your RNA is uncapped. Cap with VCE to Cap-0, then methylate to Cap-1.
Q3. What benefit does Cap-1 provide vs Cap-0?
Typically higher translation and reduced innate immune sensing
Q4. Storage and stability?
Store at −20 °C; avoid repeated freeze–thaw. Supplied in 50% glycerol buffer.
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