CVX™ Poly(A) Tailing Kit
High-efficiency poly(A) tailing kit for post-transcriptional RNA modification.
CVX™ Poly(A) Tailing Kit provides a fast and reliable method to add poly(A) tails to RNA molecules generated by in vitro transcription. The optimized formulation ensures efficient 3′-end adenylation using a recombinant E. coli Poly(A) Polymerase, improving RNA stability and translational efficiency in downstream applications.
This kit includes all necessary components for controlled polyadenylation reactions, ensuring reproducible results with minimal hands-on time. It is ideal for RNA synthesis, mRNA vaccine research, and functional RNA studies where stability and translation are critical.
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Detailed information:
Advantages & Features
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Efficient tailing: Adds long, stable poly(A) tails to transcribed RNA.
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Enhanced RNA stability: Improves translation and half-life of RNA.
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Ready-to-use components: Simplifies setup and minimizes variability.
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Optimized enzyme formulation: High activity and processivity.
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Compatible with multiple polymerases: Works with RNA generated using T7, SP6, or T3 systems.
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Reproducible performance: Consistent results across reactions.
Specifications
Includes
– 100 µl E. coli Poly(A) Polymerase (5 units/µl)
– 1 ml E. coli Poly(A) Polymerase Reaction Buffer (10X)
– 100 μl ATP (100 mM)
– 5 ml Nuclease-Free Water
Download documentation
Datasheet MSDSApplications
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Post-transcriptional RNA modification.
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Enhancement of RNA stability and translation.
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Preparation of mRNA for transfection or in vitro translation.
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Synthetic RNA production and mRNA therapeutics research.
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CRISPR guide RNA stabilization.
Tables & Figures
Quality Control
- Each batch is tested for poly(A) polymerase activity, reaction efficiency, and RNase-free integrity to ensure consistent and reproducible RNA modification performance.
Advice
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Use purified, RNase-free RNA as substrate.
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Keep all reagents on ice during setup.
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Optimize reaction time to control poly(A) tail length.
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Store enzyme mix in small aliquots to avoid repeated freeze–thaw cycles.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: Can I use RNA synthesized with other Canvax kits?
Yes. It is fully compatible with RNA produced using CVX™ T7, SP6, or T3 RNA Synthesis Kits.
Q2: What is the typical length of the poly(A) tail?
Typically between 50 and 200 adenine residues, depending on reaction time and enzyme amount.
Q3: Does the kit include control RNA?
Yes. A short control RNA is provided for reaction validation and optimization.
